Please use this identifier to cite or link to this item:
DC FieldValue
dc.titleGrowth temperature alters Salmonella Enteritidis heat/acid resistance, membrane lipid composition and stress/virulence related gene expression
dc.contributor.authorYang, Y.
dc.contributor.authorKhoo, W.J.
dc.contributor.authorZheng, Q.
dc.contributor.authorChung, H.-J.
dc.contributor.authorYuk, H.-G.
dc.identifier.citationYang, Y., Khoo, W.J., Zheng, Q., Chung, H.-J., Yuk, H.-G. (2014-02-17). Growth temperature alters Salmonella Enteritidis heat/acid resistance, membrane lipid composition and stress/virulence related gene expression. International Journal of Food Microbiology 172 : 102-109. ScholarBank@NUS Repository.
dc.description.abstractThe influence of growth temperature (10, 25, 37, and 42°C) on the survival of Salmonella Enteritidis in simulated gastric fluid (SGF; pH=2.0) and during heat treatment (54, 56, 58, and 60°C), on the membrane fatty acid composition, as well as on stress-/virulence-related gene expression was studied. Cells incubated at temperatures lower or higher than 37°C did not increase their acid resistance, with the maximum D-value of 3.07min in cells grown at 37°C; while those incubated at higher temperature increased their heat resistance, with the maximum D60°C-values of 1.4min in cells grown at 42°C. A decrease in the ratio of unsaturated to saturated fatty acids was observed as the growth temperature increased. Compared to the control cells grown at 37°C, the expression of rpoS was 16.5- and 14.4-fold higher in cells cultivated at 10 and 25°C, respectively; while the expression of rpoH was 2.9-fold higher in those cultivated at 42°C. The increased expression of stress response gene rpoH and the decreased ratio of unsaturated to saturated fatty acids correlated with the greater heat resistance of bacteria grown at 42°C; while the decreased expression of stress response gene rpoS at 42°C might contribute to the decrease in acid resistance. Virulence related genes-spvR, hilA, avrA-were induced in cells cultivated at 42°C, except sefA which was induced in the control cells. This study indicates that environmental temperature may affect the virulence potential of S. Enteritidis, thus temperature should be well controlled during food storage. © 2013 Elsevier B.V.
dc.subjectAcid resistance
dc.subjectGene expression
dc.subjectGrowth temperature
dc.subjectHeat resistance
dc.subjectMembrane lipid composition
dc.subjectSalmonella Enteritidis
dc.description.sourcetitleInternational Journal of Food Microbiology
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.