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Title: Fluorescence detection in capillary electrophoresis using a variable wavelength epi-fluorescence microscope
Authors: Chan, K.T.
Yao, Y.J.
Ng, C.L.
Li, S.F.Y. 
Issue Date: Jul-1994
Citation: Chan, K.T., Yao, Y.J., Ng, C.L., Li, S.F.Y. (1994-07). Fluorescence detection in capillary electrophoresis using a variable wavelength epi-fluorescence microscope. Fresenius' Journal of Analytical Chemistry 349 (7) : 487-490. ScholarBank@NUS Repository.
Abstract: A capillary electrophoresis fluorescence detector is described. A high-pressure mercury lamp with a filter block allowed the selection of a particular excitation waveband. Detection was performed on-column, the fluorescence emission was monitored and measured with a silicon photodiode detector with a built-in amplifier. The concentration limit of detection (CLOD) of 0.4 ng/mL was obtained for rhodamine B, a fluorescent indicator. Based on an estimated injection volume of 2.5 nL, the mass limit of detection (MLOD) was 2.1×10-18 mol. The separation of three fluorescent indicators: thionine, eosin yellowish and rhodamine B, was achieved in less than 6 min. The separation of nine porphyrin-free acids using the system developed was also demonstrated. The advantages and potential of using an epi-illumination microscope as a versatile and sensitive fluorescence detection system for capillary electrophoresis are described. © 1994 Springer-Verlag.
Source Title: Fresenius' Journal of Analytical Chemistry
ISSN: 09370633
DOI: 10.1007/BF00323979
Appears in Collections:Staff Publications

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