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Title: Evaluation of hyperbranched poly(amino ester)s of amine constitutions similar to polyethlenimine for DNA delivery
Authors: Wu, D.
Liu, Y.
Jiang, X.
Chen, L.
He, C.
Goh, S.H. 
Leong, K.W.
Issue Date: Nov-2005
Citation: Wu, D., Liu, Y., Jiang, X., Chen, L., He, C., Goh, S.H., Leong, K.W. (2005-11). Evaluation of hyperbranched poly(amino ester)s of amine constitutions similar to polyethlenimine for DNA delivery. Biomacromolecules 6 (6) : 3166-3173. ScholarBank@NUS Repository.
Abstract: New hyperbranched poly(amino ester)s were synthesized via A3 + 2BB′B″ approach, represented by the Michael addition polymerization of trimethylol-propane triacrylate (TMPTA) (A3-type monomers) with a double molar 1-(2-aminoethyl)piperazine (AEPZ) (BB′B″-type monomer) performed in chloroform at ambient temperature. The results obtained by in situ monitoring the polymerization using NMR and MS indicated that hyperbranched poly(TMPTA1-AEPZ2) was formed via a A(B′B″)2 intermediate, and the B″ (the formed 2° amine) was kept intact in the reaction. Therefore, poly(TMPTA1-AEPZ2) contained secondary and tertiary amines in the core and primary amines in the periphery similar to polyethylenimine (PEI). The chemistry of protonated poly(TMPTA1-AEPZ2) was further confirmed by 13C NMR, and the molecular weight, the radius of gyration (Rg), and the hydrodynamic radius (Rh) were determined using GPC, small-angle X-ray scattering (SAXS), and laser dynamic light scattering (LDLS), respectively. The ratio of Rg/R b of ca. 1.1 verified the hyperbranched structure. Protonated hyperbranched poly(TMPTA1-AEPZ2) is degradable and less cytotoxic as compared with PEI (25 K). Gel electrophoresis reflected that stable complexes could be formed from protonated hyperbranched poly(TMPTA1-AEPZ2) and DNA, and the size and ξ-potential of the complexes were characterized. Remarkably, protonated hyperbranched poly(TMPTA1-AEPZ2) showed transfection efficiency comparable to PEI (25 k) for in vitro DNA delivery. © 2005 American Chemical Society.
Source Title: Biomacromolecules
ISSN: 15257797
DOI: 10.1021/bm0504983
Appears in Collections:Staff Publications

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