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|Title:||Evaluation of hyperbranched poly(amino ester)s of amine constitutions similar to polyethlenimine for DNA delivery||Authors:||Wu, D.
|Issue Date:||Nov-2005||Citation:||Wu, D., Liu, Y., Jiang, X., Chen, L., He, C., Goh, S.H., Leong, K.W. (2005-11). Evaluation of hyperbranched poly(amino ester)s of amine constitutions similar to polyethlenimine for DNA delivery. Biomacromolecules 6 (6) : 3166-3173. ScholarBank@NUS Repository. https://doi.org/10.1021/bm0504983||Abstract:||New hyperbranched poly(amino ester)s were synthesized via A3 + 2BB′B″ approach, represented by the Michael addition polymerization of trimethylol-propane triacrylate (TMPTA) (A3-type monomers) with a double molar 1-(2-aminoethyl)piperazine (AEPZ) (BB′B″-type monomer) performed in chloroform at ambient temperature. The results obtained by in situ monitoring the polymerization using NMR and MS indicated that hyperbranched poly(TMPTA1-AEPZ2) was formed via a A(B′B″)2 intermediate, and the B″ (the formed 2° amine) was kept intact in the reaction. Therefore, poly(TMPTA1-AEPZ2) contained secondary and tertiary amines in the core and primary amines in the periphery similar to polyethylenimine (PEI). The chemistry of protonated poly(TMPTA1-AEPZ2) was further confirmed by 13C NMR, and the molecular weight, the radius of gyration (Rg), and the hydrodynamic radius (Rh) were determined using GPC, small-angle X-ray scattering (SAXS), and laser dynamic light scattering (LDLS), respectively. The ratio of Rg/R b of ca. 1.1 verified the hyperbranched structure. Protonated hyperbranched poly(TMPTA1-AEPZ2) is degradable and less cytotoxic as compared with PEI (25 K). Gel electrophoresis reflected that stable complexes could be formed from protonated hyperbranched poly(TMPTA1-AEPZ2) and DNA, and the size and ξ-potential of the complexes were characterized. Remarkably, protonated hyperbranched poly(TMPTA1-AEPZ2) showed transfection efficiency comparable to PEI (25 k) for in vitro DNA delivery. © 2005 American Chemical Society.||Source Title:||Biomacromolecules||URI:||http://scholarbank.nus.edu.sg/handle/10635/93774||ISSN:||15257797||DOI:||10.1021/bm0504983|
|Appears in Collections:||Staff Publications|
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