Please use this identifier to cite or link to this item: https://doi.org/10.1021/jp050625p
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dc.titleElectrochemical impedance sensing of DNA hybridization on conducting polymer film-modified diamond
dc.contributor.authorGu, H.
dc.contributor.authorSu, X.D.
dc.contributor.authorLoh, K.P.
dc.date.accessioned2014-10-16T08:27:23Z
dc.date.available2014-10-16T08:27:23Z
dc.date.issued2005-07-21
dc.identifier.citationGu, H., Su, X.D., Loh, K.P. (2005-07-21). Electrochemical impedance sensing of DNA hybridization on conducting polymer film-modified diamond. Journal of Physical Chemistry B 109 (28) : 13611-13618. ScholarBank@NUS Repository. https://doi.org/10.1021/jp050625p
dc.identifier.issn15206106
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/93701
dc.description.abstractThe impedimetric sensing of DNA hybridization on polyaniline/polyacrylate (PANI/PAA)-modified borondoped diamond (BDD) electrode has been investigated. An ultrathin film of PANI-PAA copolymer was electropolymerized onto the diamond surfaces to provide carboxylic groups for tethering to DNA sensing probes. The electrochemical impedance and the intrinsic electroactivity of the polymer-diamond interface were analyzed after the hybridization reaction with target and non-target DNA. The impedance measurement shows changes in the impedance modulus as well as electron-transfer resistance at the stage of probe DNA immobilization (single-strand), as well as after hybridization with target DNA (double-strand). DNA hybridization increases the capacitance of the polymer-DNA layer and reduces the overall impedance of the DNA-polymer-diamond stack significantly. The polymer-modified BDD electrode shows no detectable nonspecific adsorption, with good selectivity between the complementary DNA targets and the one-base mismatch targets. The detection limit was measured to be 2 × 10 -8 M at 1000 Hz. Denaturing test on the hybridized probe and subsequent reuse of the probe indicates chemical robustness of the sensor. Our results suggest that electropolymerization followed by the immobilization of biomolecules is a simple and effective way of creating a functional biomolecular scaffold on the diamond surface. In addition, label-free electrochemical impedance method can provide direct and noninvasive sensing of DNA hybridization on BDD. © 2005 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/jp050625p
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1021/jp050625p
dc.description.sourcetitleJournal of Physical Chemistry B
dc.description.volume109
dc.description.issue28
dc.description.page13611-13618
dc.description.codenJPCBF
dc.identifier.isiut000230526800031
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