Please use this identifier to cite or link to this item: https://doi.org/10.1002/adfm.200800675
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dc.titleAnatomy and growth characteristics of conjugated polyelectrolyte/DNA aggregates
dc.contributor.authorChi, C.
dc.contributor.authorChworos, A.
dc.contributor.authorZhang, J.
dc.contributor.authorMikhailovsky, A.
dc.contributor.authorBazan, G.C.
dc.date.accessioned2014-10-16T08:20:24Z
dc.date.available2014-10-16T08:20:24Z
dc.date.issued2008-11-24
dc.identifier.citationChi, C., Chworos, A., Zhang, J., Mikhailovsky, A., Bazan, G.C. (2008-11-24). Anatomy and growth characteristics of conjugated polyelectrolyte/DNA aggregates. Advanced Functional Materials 18 (22) : 3606-3612. ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.200800675
dc.identifier.issn1616301X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/93101
dc.description.abstractConjugated polyelectrolytes (CPEs) have been widely used as light harvesting macromolecules to amplify the signals of fluorescent assays that betray the presence of various biomolecular targets. Electrostatic interactions play an important role in coordinating optical coupling events and lead to the formation of complexes between oppositely charged CPEs and the target species. Here, we combine for the first time optical studies and structural characterization by liquid phase atomic force microscopy (AFM) to provide a picture of aggregate structure and growth dynamics between cationic CPE and DNA as a function of charge ratio. Specifically, Poly120, a copolymer containing a backbone with 50% fluorene, 30% phenylene, and 20% 2,1,3-benzothiadiazole (BT) units and pendant cationic groups was mixed with single stranded DNA (ssDNA) labeled with the Cy5 acceptor chromophore. Continuous addition of ssDNA-Cy5 to poly120 leads to a saturation in Cy5 emission due to Cy5-Cy5 self-quenching. Addition of ssDNA to a preformed poly120/ssDNA-Cy5 solution results in increased sensitization by energy transfer and a reduction of Cy5-Cy5 self quenching. Adsorption of aggregates onto negatively charged mica under water allows for direct imaging of the polyelectrolyte complexes as a function of charge ratio. The composite set of observations allows for the development of a model for aggregate growth, which is dynamic and ceases when the surface charge becomes sufficiently negative. This saturation point can be mitigated by addition of unlabeled ssDNA. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA,.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/adfm.200800675
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1002/adfm.200800675
dc.description.sourcetitleAdvanced Functional Materials
dc.description.volume18
dc.description.issue22
dc.description.page3606-3612
dc.description.codenAFMDC
dc.identifier.isiut000261484000009
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