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https://scholarbank.nus.edu.sg/handle/10635/92027
DC Field | Value | |
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dc.title | High density of immobilized galactose ligand enhances hepatocyte attachment and function | |
dc.contributor.author | Yin, C. | |
dc.contributor.author | Ying, L. | |
dc.contributor.author | Zhang, P.-C. | |
dc.contributor.author | Zhuo, R.-X. | |
dc.contributor.author | Kang, E.-T. | |
dc.contributor.author | Leong, K.W. | |
dc.contributor.author | Mao, H.-Q. | |
dc.date.accessioned | 2014-10-09T09:54:31Z | |
dc.date.available | 2014-10-09T09:54:31Z | |
dc.date.issued | 2003-12-15 | |
dc.identifier.citation | Yin, C.,Ying, L.,Zhang, P.-C.,Zhuo, R.-X.,Kang, E.-T.,Leong, K.W.,Mao, H.-Q. (2003-12-15). High density of immobilized galactose ligand enhances hepatocyte attachment and function. Journal of Biomedical Materials Research - Part A 67 (4) : 1093-1104. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00219304 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/92027 | |
dc.description.abstract | Galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes. In this study, we described a scheme to achieve high density of immobilized galactose ligands on polyethylene terephthalate (PET) surface by first surface-grafting polyacrylic acid on plasma-pretreated PET film under UV irradiation, followed by conjugation of a galactose derivative (1-O-(6′-aminohexyl)-D-galactopyranoside) to the grafted polyacrylic acid chains. A high galactose density of 513 nmol/cm 2 on the PET surface was used in this study to investigate the behavior of cultured hepatocyte. This engineered substrate showed high affinity to fluorescein isothiocyanate-lectin binding. Primary rat hepatocytes, when seeded at a density of 2 x 105 cells/cm2, attached to the galactosylated PET substrate at a similar efficiency compared with collagen-coated substrate. The hepatocytes spontaneously formed aggregates 1 day after cell seeding and showed better maintenance of albumin secretion and urea synthesis functions than those cultured on collagen-coated surface. © 2003 Wiley Periodicals, Inc. | |
dc.source | Scopus | |
dc.subject | Function maintenance | |
dc.subject | Galactosylated surface | |
dc.subject | Hepatocyte | |
dc.subject | Immobilization | |
dc.subject | Ligand | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & ENVIRONMENTAL ENGINEERING | |
dc.description.sourcetitle | Journal of Biomedical Materials Research - Part A | |
dc.description.volume | 67 | |
dc.description.issue | 4 | |
dc.description.page | 1093-1104 | |
dc.description.coden | JBMRC | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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