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Title: High density of immobilized galactose ligand enhances hepatocyte attachment and function
Authors: Yin, C.
Ying, L.
Zhang, P.-C.
Zhuo, R.-X.
Kang, E.-T. 
Leong, K.W.
Mao, H.-Q.
Keywords: Function maintenance
Galactosylated surface
Issue Date: 15-Dec-2003
Citation: Yin, C.,Ying, L.,Zhang, P.-C.,Zhuo, R.-X.,Kang, E.-T.,Leong, K.W.,Mao, H.-Q. (2003-12-15). High density of immobilized galactose ligand enhances hepatocyte attachment and function. Journal of Biomedical Materials Research - Part A 67 (4) : 1093-1104. ScholarBank@NUS Repository.
Abstract: Galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes. In this study, we described a scheme to achieve high density of immobilized galactose ligands on polyethylene terephthalate (PET) surface by first surface-grafting polyacrylic acid on plasma-pretreated PET film under UV irradiation, followed by conjugation of a galactose derivative (1-O-(6′-aminohexyl)-D-galactopyranoside) to the grafted polyacrylic acid chains. A high galactose density of 513 nmol/cm 2 on the PET surface was used in this study to investigate the behavior of cultured hepatocyte. This engineered substrate showed high affinity to fluorescein isothiocyanate-lectin binding. Primary rat hepatocytes, when seeded at a density of 2 x 105 cells/cm2, attached to the galactosylated PET substrate at a similar efficiency compared with collagen-coated substrate. The hepatocytes spontaneously formed aggregates 1 day after cell seeding and showed better maintenance of albumin secretion and urea synthesis functions than those cultured on collagen-coated surface. © 2003 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part A
ISSN: 00219304
Appears in Collections:Staff Publications

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