Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1008347630546
DC FieldValue
dc.titleFacilitation of cometabolic degradation of 4-chlorophenol using glucose as an added growth substrate
dc.contributor.authorWang, S.-J.
dc.contributor.authorLoh, K.-C.
dc.date.accessioned2014-10-09T09:53:44Z
dc.date.available2014-10-09T09:53:44Z
dc.date.issued1999
dc.identifier.citationWang, S.-J., Loh, K.-C. (1999). Facilitation of cometabolic degradation of 4-chlorophenol using glucose as an added growth substrate. Biodegradation 10 (4) : 261-269. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1008347630546
dc.identifier.issn09239820
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/91989
dc.description.abstractThis paper reports on the feasibility of using glucose as an added substrate for cometabolic transformation of 4-chlorophenol (4-cp). When glucose was fed as the added growth substrate, only 78% and 43% of the initial 4-cp concentrations of 100 and 200 mg l-1, respectively, were transformed before the pH dropped to below 4.5 and stopped all reactions. By maintaining the medium pH, complete removal of 4-cp was achieved even at the high initial concentration of 200 mg l-1. Phenol induction prior to inoculation was not a prerequisite to ensure transformation of 4-cp when glucose was the added growth substrate. Compared with phenol as the added growth substrate, cells grown on glucose displayed a longer acclimation phase and, in general, a lower specific transformation rate. The volumetric transformation rate of 4-cp, however, was greatly enhanced due to the increased cell density. The results of this work suggest that 4-cp itself induced the enzymes necessary for its cometabolism. With NADH regenerated effectively through metabolism of glucose, 4-cp was transformed in the absence of added phenol. Consequently, the competitive inhibition involved in cometabolism was avoided and the risks associated with addition of toxic growth substrates such as phenol were eliminated.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1023/A:1008347630546
dc.sourceScopus
dc.subject4-chlorophenol
dc.subjectBiotransformation
dc.subjectCometabolism
dc.subjectGlucose
dc.subjectGrowth substrate
dc.subjectInhibition
dc.subjectPH regulation
dc.subjectPhenol
dc.subjectToxicity
dc.typeArticle
dc.contributor.departmentCHEMICAL & ENVIRONMENTAL ENGINEERING
dc.description.doi10.1023/A:1008347630546
dc.description.sourcetitleBiodegradation
dc.description.volume10
dc.description.issue4
dc.description.page261-269
dc.description.codenBIODE
dc.identifier.isiut000084739600004
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

77
checked on Nov 29, 2022

WEB OF SCIENCETM
Citations

68
checked on Nov 29, 2022

Page view(s)

126
checked on Nov 24, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.