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Title: Effects of Singapore soil type on bioavailability of nutrients in soil bioremediation
Authors: Kwok, C.-K.
Loh, K.-C. 
Keywords: Bioavailability
Pseudomonas putida
Issue Date: Jun-2003
Citation: Kwok, C.-K., Loh, K.-C. (2003-06). Effects of Singapore soil type on bioavailability of nutrients in soil bioremediation. Advances in Environmental Research 7 (4) : 889-900. ScholarBank@NUS Repository.
Abstract: The biodegradation of phenanthrene by Pseudomonas putida (ATCC 17484) was studied in six different synthetic soils representing the chemical compositions of six Singapore soil types. The nutrients used were ammonium nitrate to provide nitrogen, potassium dihydrogen phosphate (KH2PO4) and phenyl phosphate (PP) disodium salt to provide phosphorus. Sorption isotherms of the nutrients in each soil type were determined. Bioavailability of nutrients is measured by the ratio of the rate of biodegradation in the presence of nutrients to that in the absence of nutrients. The bioavailability was found to correspond closely to the KH2PO4 sorption capacity of a soil. Increasing KH2PO4 increased the biodegradation rate of phenanthrene across all soil types, indicating that phosphorus was limiting. The organic phosphorus compound, PP, has greater sorption ability than KH2PO4. It was tested as a substitute for KH2PO4 in the biodegradation of phenanthrene and was found to increase the bioavailability of phosphorus. It is proposed that bacteria attach themselves on the external surfaces of soil particles and utilize the nutrients sorbed there. The bioavailability of phosphorus from both KH2PO4 and PP was well correlated with the effective concentration of phosphorus-defined by the product of the equilibrium concentration of phosphorus sorbed in a soil and the fraction of external surface area over total surface area of soil particles. The Monod model fits the above correlation reasonably well. This study can be applied to the bioremediation of local soils in determining the amount of nutrients to use. © 2002 Elsevier Science Ltd. All rights reserved.
Source Title: Advances in Environmental Research
ISSN: 10930191
DOI: 10.1016/S1093-0191(02)00084-9
Appears in Collections:Staff Publications

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