Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0021-9673(96)00779-0
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dc.titleRole of polyethyleneimine in the purification of recombinant human tumour necrosis factor beta
dc.contributor.authorLoh, K.C.
dc.contributor.authorYao, Z.J.
dc.contributor.authorYap, M.G.S.
dc.contributor.authorChung, M.C.M.
dc.date.accessioned2014-10-09T08:21:02Z
dc.date.available2014-10-09T08:21:02Z
dc.date.issued1997-01-31
dc.identifier.citationLoh, K.C., Yao, Z.J., Yap, M.G.S., Chung, M.C.M. (1997-01-31). Role of polyethyleneimine in the purification of recombinant human tumour necrosis factor beta. Journal of Chromatography A 760 (2) : 165-171. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(96)00779-0
dc.identifier.issn00219673
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/91687
dc.description.abstractThe chromatographic behaviour of recombinant human tumour necrosis factor beta (rhTNF-β) (pI ~9.0) during cation-exchange chromatography at pH 7.5 is investigated. Without prior treatment of the Escherichia coli cell extract with polyethyleneimine (PEI), very little rhTNF-β was bound to the column. However, upon addition of 5% PEI (100 μl ml-1) to the cell lysate, rhTNF-β was shown to bind to cation-exchange columns normally. TNF-β was readily precipitated from the clarified cell extract by 20% ammonium sulphate, but only ca. 25% of this precipitate could be re-solubilized for further purification. However, when 5% PEI was included in the solubilization buffer, the balance of the rhTNF-β could be recovered. It is proposed that charge interaction between rhTNF-β and nucleic acids in the cell extract is responsible for both of these anomalous phenomena, and that PEI (a cationic polyelectrolyte) was able to disrupt this interaction by displacing rhTNF-β from the charge complex.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0021-9673(96)00779-0
dc.sourceScopus
dc.subjectPolyethyleneimine
dc.subjectProteins
dc.subjectTumor necrosis factor
dc.typeArticle
dc.contributor.departmentBIOPROCESSING TECHNOLOGY CENTRE
dc.contributor.departmentCHEMICAL ENGINEERING
dc.description.doi10.1016/S0021-9673(96)00779-0
dc.description.sourcetitleJournal of Chromatography A
dc.description.volume760
dc.description.issue2
dc.description.page165-171
dc.description.codenJCRAE
dc.identifier.isiutA1997WK10100001
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