Please use this identifier to cite or link to this item: https://doi.org/10.1002/(SICI)1097-0290(19980605)58:53.0.CO;2-H
DC FieldValue
dc.titleGenetically structured mathematical modeling of trp attenuator mechanism
dc.contributor.authorKoh, B.T.
dc.contributor.authorTan, R.B.H.
dc.contributor.authorYap, M.G.S.
dc.date.accessioned2014-10-09T08:18:40Z
dc.date.available2014-10-09T08:18:40Z
dc.date.issued1998-06-05
dc.identifier.citationKoh, B.T.,Tan, R.B.H.,Yap, M.G.S. (1998-06-05). Genetically structured mathematical modeling of trp attenuator mechanism. Biotechnology and Bioengineering 58 (5) : 502-509. ScholarBank@NUS Repository. <a href="https://doi.org/10.1002/(SICI)1097-0290(19980605)58:53.0.CO;2-H" target="_blank">https://doi.org/10.1002/(SICI)1097-0290(19980605)58:53.0.CO;2-H</a>
dc.identifier.issn00063592
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/91489
dc.description.abstractA genetically structured mathematical model of the trp attenuator in Escherichia coli based on known coupling mechanisms of the transcription of the trp leader region and translation of the trp leader peptide region is proposed. The model simulates, both qualitatively and quantitatively, the effects of tryptophan on the repression of cloned gene products. It shows that repression by attenuation mechanism alone operates over a narrow trp concentration range of 1 to 5 μM compared with 1 to 100 μM for trp repressor mechanism. This implies that attenuation by transcription termination is not relaxed until tryptophan starvation is severe. Simulation results show that the attenuator starts to derepress when the repressor is about 40% repressed, and becomes significantly derepressed only when the repressor repression decreased to about 20%. Unlike the case of repressor- operator interaction, the operating range of tryptophan concentration in the attenuator mechanism is not sensitive to plasmid copy number.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/(SICI)1097-0290(19980605)58:53.0.CO;2-H
dc.sourceScopus
dc.subjectCloned gene expression control
dc.subjectGenetically structured mathematical model
dc.subjectTrp operon
dc.typeArticle
dc.contributor.departmentCHEMICAL ENGINEERING
dc.contributor.departmentBIOPROCESSING TECHNOLOGY CENTRE
dc.description.doi10.1002/(SICI)1097-0290(19980605)58:53.0.CO;2-H
dc.description.sourcetitleBiotechnology and Bioengineering
dc.description.volume58
dc.description.issue5
dc.description.page502-509
dc.description.codenBIBIA
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

50
checked on May 21, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.