Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/91442
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dc.titleDiffusional kinetics of monoclonal antibody, transferrin and bovine serum albumin on Sephacryl S-200 HR gel
dc.contributor.authorChing, C.B.
dc.contributor.authorUddin, M.S.
dc.contributor.authorHidajat, K.
dc.date.accessioned2014-10-09T08:18:08Z
dc.date.available2014-10-09T08:18:08Z
dc.date.issued1989-12
dc.identifier.citationChing, C.B.,Uddin, M.S.,Hidajat, K. (1989-12). Diffusional kinetics of monoclonal antibody, transferrin and bovine serum albumin on Sephacryl S-200 HR gel. The Chemical Engineering Journal 42 (3) : B47-B52. ScholarBank@NUS Repository.
dc.identifier.issn03009467
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/91442
dc.description.abstractThe adsorption and diffusional kinetics of three macromolecules, namely bovine serum albumin (BSA), transferrin and monoclonal antibody (McAb), on Sephacryl S-200 high resolution gel were studied by the pulse-response chromatographic technique in a column of diameter 1.6 cm and length 8.5 cm. Experimental results showed that the voidage ε and axial mixing (2DL/V) in the packed column were 0.432 and 0.05 cm based on the response to a pulse input of blue dextran 2 000 000 and D2O solution respectively. The equilibrium constant K and pore diffusivity Dp were 0.23 and (0.60 - 1.02) × 10-7 cm2 s-1 for BSA (molecular weight, 67 000) 0.17 and (0.62 - 1.0) × 10-7 cm2 s-1 for transferrin (molecular weight, 95 000) and 0.095 and (0.53 - 0.85) × 10-7 cm2 s-1 for McAb (molecular weight, 150 000 IgG). A van't Hoff plot of ln K vs. 1/T gave a straight line with zero slope and hence zero heat of sorption for all three components. This implies that no adsorption occurs between the molecules and the packing, and that the partition of solute in the bulk solution and pore solution is entirely controlled by diffusional forces. © 1989.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMICAL ENGINEERING
dc.description.sourcetitleThe Chemical Engineering Journal
dc.description.volume42
dc.description.issue3
dc.description.pageB47-B52
dc.identifier.isiutNOT_IN_WOS
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