Please use this identifier to cite or link to this item: https://doi.org/10.1002/jcb.24105
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dc.titleIntramuscular nerve damage in lacerated skeletal muscles may direct the inflammatory cytokine response during recovery
dc.contributor.authorPereira, B.P.
dc.contributor.authorTan, B.L.
dc.contributor.authorHan, H.C.
dc.contributor.authorZou, Y.
dc.contributor.authorAung, K.Z.
dc.contributor.authorLeong, D.T.
dc.date.accessioned2014-10-09T07:09:44Z
dc.date.available2014-10-09T07:09:44Z
dc.date.issued2012-07
dc.identifier.citationPereira, B.P., Tan, B.L., Han, H.C., Zou, Y., Aung, K.Z., Leong, D.T. (2012-07). Intramuscular nerve damage in lacerated skeletal muscles may direct the inflammatory cytokine response during recovery. Journal of Cellular Biochemistry 113 (7) : 2330-2345. ScholarBank@NUS Repository. https://doi.org/10.1002/jcb.24105
dc.identifier.issn07302312
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/90845
dc.description.abstractThe expression of inflammatory cytokines and growth factors in surgically repaired lacerated muscles over a 12-week recovery phase was investigated. We hypothesized that these expression levels are influenced by both neural and muscular damage within lacerated muscles. Microarrays were confirmed with reverse transcription-polymerase chain reaction assays and histology of biopsies at the lesion of three simulated lacerated muscle models in 130 adult rats. The lacerated medial gastrocnemius with the main intramuscular nerve branch either cut (DN), crushed but leaving an intact nerve sheath (RN); or preserved intact (PN) were compared. At 4 weeks, DN had a higher number of interleukins up-regulated. DN and RN also had a set of Bmp genes significantly expressed between 2 and 8 weeks (Pâ‰̂‰0.05). By 12 weeks, DN had a poorer and slower myogenic recovery and greater fibrosis formation correlating with an up-regulation of the Tgf-β gene family. DN also showed poorer re-innervation with higher mRNA expression levels of nerve growth factor (Ngf) and brain-derived neurotrophin growth factor (Bdnf) over RN and PN. This study demonstrates that the inflammatory response over 12 weeks in lacerated muscles may be directed by the type of intramuscular nerve damage, which can influence the recovery at the lesion site. Inflammatory-related genes associated to the type of intramuscular nerve damage include Gas-6, Artemin, Fgf10, Gdf8, Cntf, Lif, and Igf-2. qPCR also found up-regulation of Bdnf (1-week), neurotrophin-3 (2w), Lif (4w), and Ngf (4w, 8w) mRNA expressions in DN, making them possible candidates for therapeutic treatment to arrest the poor recovery in muscle lacerations (250). © 2012 Wiley Periodicals, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/jcb.24105
dc.sourceScopus
dc.subjectOLIGO cDNA microarray
dc.subjectquantitative reverse-transcription polymerase chain reaction
dc.subjectre-innervation
dc.typeReview
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1002/jcb.24105
dc.description.sourcetitleJournal of Cellular Biochemistry
dc.description.volume113
dc.description.issue7
dc.description.page2330-2345
dc.description.codenJCEBD
dc.identifier.isiut000303797600017
Appears in Collections:Staff Publications

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