Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jcis.2010.03.004
Title: Synthesis and characterization of β-cyclodextrin-conjugated magnetic nanoparticles and their uses as solid-phase artificial chaperones in refolding of carbonic anhydrase bovine
Authors: Badruddoza, A.Z.M.
Hidajat, K. 
Uddin, M.S. 
Keywords: β-Cyclodextrin
Artificial chaperone
Carbonic anhydrase
Magnetic nanoparticles
Protein refolding
Issue Date: Jun-2010
Citation: Badruddoza, A.Z.M., Hidajat, K., Uddin, M.S. (2010-06). Synthesis and characterization of β-cyclodextrin-conjugated magnetic nanoparticles and their uses as solid-phase artificial chaperones in refolding of carbonic anhydrase bovine. Journal of Colloid and Interface Science 346 (2) : 337-346. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jcis.2010.03.004
Abstract: Surface-functionalized magnetic nanoparticles are widely used in various fields of biotechnology. In this study, β-cyclodextrin-conjugated magnetic nanoparticles (CD-APES-MNPs) are synthesized and the use of CD-APES-MNPs as a solid-phase artificial chaperone to assist protein refolding in vitro is demonstrated using carbonic anhydrase bovine (CA) as model protein. CD-APES-MNPs are fabricated by grafting mono-tosyl-β-cyclodextrin (Ts-β-CD) onto 3-aminopropyltriethoxysilane (APES)-modified magnetic nanoparticles (APES-MNPs). Results obtained from transmission electron microscopy (TEM) and vibrating sample magnetometery (VSM) show that the synthesized magnetic nanoparticles are superparamagnetic with a mean diameter of 11.5nm. The β-CD grafting is confirmed by Fourier transform infrared spectroscopy (FTIR) and elemental analysis. The amount of β-CD grafted on the APES-MNPs is found to be 0.042mmolg-1 from elemental analysis. Our refolding results show that a maximum of 85% CA refolding yield can be achieved using these β-CD-conjugated magnetic nanoparticles which is at the same level as that using liquid-phase artificial chaperone-assisted refolding. In addition, the secondary and tertiary structures of the refolded CA are the same as those of native protein under optimal conditions. These results indicate that CD-APES-MNPs are suitable and efficient stripping agents for solid-phase artificial chaperone-assisted refolding due to easier and faster separation of these nanoparticles from the refolded samples and also due to recycling of the stripping agents. © 2010 Elsevier Inc.
Source Title: Journal of Colloid and Interface Science
URI: http://scholarbank.nus.edu.sg/handle/10635/90263
ISSN: 00219797
DOI: 10.1016/j.jcis.2010.03.004
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

51
checked on Nov 19, 2019

WEB OF SCIENCETM
Citations

45
checked on Jul 10, 2019

Page view(s)

52
checked on Oct 26, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.