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|Title:||One-step UV lithography for activation of inert hydrocarbon monolayers and preparation of protein micropatterns||Authors:||Xue, C.-Y.
|Issue Date:||1-Apr-2010||Citation:||Xue, C.-Y., Yang, K.-L. (2010-04-01). One-step UV lithography for activation of inert hydrocarbon monolayers and preparation of protein micropatterns. Journal of Colloid and Interface Science 344 (1) : 48-53. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jcis.2009.12.015||Abstract:||Materials with chemical micropatterned surface have broad applications in many fields. In this article, we report a simple one-step UV lithography method to activate inert hydrocarbon monolayers for spontaneous formation of water and covalently immobilized protein micropatterns on the surface. Two types of hydrocarbon monolayers including octadecyltrichlorosilane (OTS) on silicon oxide and 1-hexadecene on hydrogen-terminated silicon were studied. It was found that after UV modifications, water can form micropatterns spontaneously on both surfaces. Furthermore, when protein solutions were used, covalently immobilized protein micropatterns can be formed. Our XPS results and controlled reducing experiments showed that UV exposure transformed inert monolayers into active surfaces with aldehyde groups, which are responsible for the covalent immobilization of proteins. The method reported herein can be applied under ambient conditions without having a high vacuum system. It can also be extended to pattern other biomolecules bearing amine groups. © 2009 Elsevier Inc. All rights reserved.||Source Title:||Journal of Colloid and Interface Science||URI:||http://scholarbank.nus.edu.sg/handle/10635/89649||ISSN:||00219797||DOI:||10.1016/j.jcis.2009.12.015|
|Appears in Collections:||Staff Publications|
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