Please use this identifier to cite or link to this item:
|Title:||Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid||Authors:||Ding, X.
|Issue Date:||15-May-2012||Citation:||Ding, X., Zhang, W., Cheng, D., He, J., Yang, K.-L. (2012-05-15). Oligopeptides functionalized surface plasmon resonance biosensors for detecting thiacloprid and imidacloprid. Biosensors and Bioelectronics 35 (1) : 271-276. ScholarBank@NUS Repository.||Abstract:||By using phage display library, we identified two highly specific oligopeptide sequences RKRIRRMMPRPS and RNRHTHLRTRPR for binding neonicotinoids such as thiacloprid and imidacloprid. The former shows high affinity for thiacloprid whereas the latter shows high affinity for imidacloprid. Surprisingly, cross binding is minimal despite the similarity of the two molecules. To develop a neonicotinoid biosensor, these two oligopeptides are synthesized and immobilized on the surface of a surface plasmon resonance (SPR) chip with a bare-gold surface. This oligopeptide functionalized SPR biosensor can rapidly detect thiacloprid and imidacloprid in buffer solutions in a real-time manner. The limit of detection (LOD) for thiacloprid and imidacloprid is 1.2 μM and 0.9 μM, respectively. © 2012 Elsevier B.V.||Source Title:||Biosensors and Bioelectronics||URI:||http://scholarbank.nus.edu.sg/handle/10635/89636||ISSN:||09565663|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 27, 2018
WEB OF SCIENCETM
checked on Dec 31, 2018
checked on Nov 30, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.