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Title: Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity
Authors: Qin, W.J. 
Yung, L.Y.L. 
Issue Date: Sep-2007
Citation: Qin, W.J., Yung, L.Y.L. (2007-09). Nanoparticle-based detection and quantification of DNA with single nucleotide polymorphism (SNP) discrimination selectivity. Nucleic Acids Research 35 (17) : -. ScholarBank@NUS Repository.
Abstract: Sequence-specific DNA detection is important in various biomedical applications such as gene expression profiling, disease diagnosis and treatment, drug discovery and forensic analysis. Here we report a gold nanoparticle-based method that allows DNA detection and quantification and is capable of single nucleotide polymorphism (SNP) discrimination. The precise quantification of single-stranded DNA is due to the formation of defined nanoparticle-DNA conjugate groupings in the presence of target/linker DNA. Conjugate groupings were characterized and quantified by gel electrophoresis. A linear correlation between the amount of target DNA and conjugate groupings was found. For SNP detection, single base mismatch discrimination was achieved for both the end- and center-base mismatch. The method described here may be useful for the development of a simple and quantitative DNA detection assay. © 2007 The Author(s).
Source Title: Nucleic Acids Research
ISSN: 03051048
DOI: 10.1093/nar/gkm602
Appears in Collections:Staff Publications

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