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Title: Minimizing nonspecific protein adsorption in liquid crystal immunoassays by using surfactants
Authors: Zhang, W.
Ang, W.T.
Xue, C.-Y.
Yang, K.-L. 
Keywords: immunoassay
interference colors
liquid crystals
nonspecific protein adsorption
Tween 20
Issue Date: 28-Sep-2011
Citation: Zhang, W., Ang, W.T., Xue, C.-Y., Yang, K.-L. (2011-09-28). Minimizing nonspecific protein adsorption in liquid crystal immunoassays by using surfactants. ACS Applied Materials and Interfaces 3 (9) : 3496-3500. ScholarBank@NUS Repository.
Abstract: In this paper, we report the role of surfactants in minimizing nonspecific protein adsorption in liquid crystal (LC)-based immunoassays in which LC is used as a readout system. Among all surfactants tested, only nonionic surfactant such as Tween 20 can effectively reduce the nonspecific protein adsorption, while maintaining the selectivity of the LC-based immunoassay. We also show that to minimize nonspecific protein adsorption, Tween 20 can be added directly into the antibody solution to a final concentration of 0.8 mM. After the addition of Tween 20, better correlations between the antibody concentrations and the interference colors of LCs can therefore be obtained. For example, when Cy3 antibiotin was used, black, yellow, red, and green interference colors correspond to a concentration of 5, 25, 50, and 100 μg/mL, respectively. This feature gives LC immunoassay a unique advantage over the fluorescence-based immunoassay. © 2011 American Chemical Society.
Source Title: ACS Applied Materials and Interfaces
ISSN: 19448244
DOI: 10.1021/am200716x
Appears in Collections:Staff Publications

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