Please use this identifier to cite or link to this item: https://doi.org/10.1186/1475-2859-13-61
DC FieldValue
dc.titleGenome-scale metabolic network reconstruction and in silico flux analysis of the thermophilic bacterium Thermus thermophilus HB27
dc.contributor.authorLee, N.-R.
dc.contributor.authorLakshmanan, M.
dc.contributor.authorAggarwal, S.
dc.contributor.authorSong, J.-W.
dc.contributor.authorKarimi, I.A.
dc.contributor.authorLee, D.-Y.
dc.contributor.authorPark, J.-B.
dc.date.accessioned2014-10-09T06:48:51Z
dc.date.available2014-10-09T06:48:51Z
dc.date.issued2014-04-28
dc.identifier.citationLee, N.-R., Lakshmanan, M., Aggarwal, S., Song, J.-W., Karimi, I.A., Lee, D.-Y., Park, J.-B. (2014-04-28). Genome-scale metabolic network reconstruction and in silico flux analysis of the thermophilic bacterium Thermus thermophilus HB27. Microbial Cell Factories 13 (1) : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1475-2859-13-61
dc.identifier.issn14752859
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/89004
dc.description.abstractBackground: Thermus thermophilus, an extremely thermophilic bacterium, has been widely recognized as a model organism for studying how microbes can survive and adapt under high temperature environment. However, the thermotolerant mechanisms and cellular metabolism still remains mostly unravelled. Thus, it is highly required to consider systems biological approaches where T. thermophilus metabolic network model can be employed together with high throughput experimental data for elucidating its physiological characteristics under such harsh conditions.Results: We reconstructed a genome-scale metabolic model of T. thermophilus, iTT548, the first ever large-scale network of a thermophilic bacterium, accounting for 548 unique genes, 796 reactions and 635 unique metabolites. Our initial comparative analysis of the model with Escherichia coli has revealed several distinctive metabolic reactions, mainly in amino acid metabolism and carotenoid biosynthesis, producing relevant compounds to retain the cellular membrane for withstanding high temperature. Constraints-based flux analysis was, then, applied to simulate the metabolic state in glucose minimal and amino acid rich media. Remarkably, resulting growth predictions were highly consistent with the experimental observations. The subsequent comparative flux analysis under different environmental conditions highlighted that the cells consumed branched chain amino acids preferably and utilized them directly in the relevant anabolic pathways for the fatty acid synthesis. Finally, gene essentiality study was also conducted via single gene deletion analysis, to identify the conditional essential genes in glucose minimal and complex media.Conclusions: The reconstructed genome-scale metabolic model elucidates the phenotypes of T. thermophilus, thus allowing us to gain valuable insights into its cellular metabolism through in silico simulations. The information obtained from such analysis would not only shed light on the understanding of physiology of thermophiles but also helps us to devise metabolic engineering strategies to develop T. thermophilus as a thermostable microbial cell factory. © 2014 Lee et al.; licensee BioMed Central Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1186/1475-2859-13-61
dc.sourceScopus
dc.subjectConstraints-based flux analysis
dc.subjectEthanol
dc.subjectGenome-scale metabolic model
dc.subjectThermophile
dc.subjectThermus thermophilus
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1186/1475-2859-13-61
dc.description.sourcetitleMicrobial Cell Factories
dc.description.volume13
dc.description.issue1
dc.description.page-
dc.description.codenMCFIC
dc.identifier.isiut000335346100001
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2014-genome_scale_metabolic_network-published.pdf1.47 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

11
checked on Oct 19, 2019

WEB OF SCIENCETM
Citations

11
checked on Oct 11, 2019

Page view(s)

90
checked on Oct 12, 2019

Download(s)

3
checked on Oct 12, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.