Please use this identifier to cite or link to this item:
|Title:||Energy transfer between conjugated-oligoelectrolyte-substituted POSS and gold nanocluster for multicolor intracellular detection of mercury ion||Authors:||Pu, K.-Y.
|Issue Date:||7-Jul-2011||Citation:||Pu, K.-Y., Luo, Z., Li, K., Xie, J., Liu, B. (2011-07-07). Energy transfer between conjugated-oligoelectrolyte-substituted POSS and gold nanocluster for multicolor intracellular detection of mercury ion. Journal of Physical Chemistry C 115 (26) : 13069-13075. ScholarBank@NUS Repository. https://doi.org/10.1021/jp203133t||Abstract:||Although metal nanoclusters (NCs) with sizes close to the Fermi wavelength of electrons are well-known for their molecule-like luminescent behaviors, their energy-transfer properties remain unrevealed. Herein, fluorescence resonance energy transfer (FRET) between blue-fluorescent conjugated-oligomer-substituted polyhedral oligomeric silsesquioxane (POSSFF) and red-fluorescent gold NCs (R-AuNCs) is investigated and subsequently utilized for mercury-ion sensing both in solution and in cell. By virtue of their opposite charges and good spectral overlap, efficient FRET from POSSFF to R-AuNC occurs upon electrostatic complex formation, leading to dual-emissive pink fluorescence upon donor excitation. The pink fluorescence specifically turns blue in the presence of mercury ions rather than other metal ions because of the strong metallophilic Hg 2+/Au+ interaction that quenches the red fluorescence from R-AuNCs. This consequently allows for visual detection and precise quantification of mercury ions with a limit of detection of ∼0.1 nM in aqueous solution. Moreover, the whole-cell permeability of the complexes and the preserved ion-selective FRET in cells make these complexes effective for multicolor intracellular sensing of mercury ion. This study thus not only develops a promising mercury-ion nanoprobe for diagnostic and clinical applications but also provides fundamental guidelines for the design of metal-NC-based intracellular nanosensors. © 2011 American Chemical Society.||Source Title:||Journal of Physical Chemistry C||URI:||http://scholarbank.nus.edu.sg/handle/10635/88838||ISSN:||19327447||DOI:||10.1021/jp203133t|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 17, 2019
WEB OF SCIENCETM
checked on Sep 10, 2019
checked on Sep 7, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.