Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.21334
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dc.titleEncapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor cone-jet mode
dc.contributor.authorXie, J.
dc.contributor.authorWang, C.-H.
dc.date.accessioned2014-10-09T06:46:52Z
dc.date.available2014-10-09T06:46:52Z
dc.date.issued2007-08-01
dc.identifier.citationXie, J., Wang, C.-H. (2007-08-01). Encapsulation of proteins in biodegradable polymeric microparticles using electrospray in the Taylor cone-jet mode. Biotechnology and Bioengineering 97 (5) : 1278-1290. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.21334
dc.identifier.issn00063592
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/88837
dc.description.abstractSolvent extraction (or evaporation from a W1/O/W 2-dispersion), coacervation, and spray drying methods are commonly employed to encapsulate protein drugs in polymeric microparticles for sustained delivery applications. To overcome the limitations of these methods, a novel etectrospray method was developed to encapsulate a model protein drug - bovine serum albumin (BSA) in biodegradable polymeric microparticles and examine the feasibility of the process in not denaturing the protein. Microparticles of approximately 20 μm diameter with corrugated surfaces and smooth surfaces were observed by scanning electron microscope. Confocal laser scanning microscope images showed that BSA was distributed evenly in microparticles. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was employed to investigate the protein integrity of BSA released from the polymer matrix after 38 days. No protein degradation was observed during the 38 days release. The secondary structure of released BSA was characterized by Fourier transform infrared (FTIR) and circular dichroism (CD), which suggested that the released BSA was almost identical to native BSA. The encapsulation efficiency could reach 76% by adjusting the amount of the additive Pluronic F127 and processing parameters. The release profile could be tailored by the fabrication process and the sustained release of BSA could endure for more than 1 month. More than 80% of the bioactivity of BSA (evaluated by BSA ELISA kit) could be maintained after releasing from polymer matrix. Findings of the present study demonstrate that this novel electrospray method is a promising approach to encapsulate bioactive materials such as proteins, enzymes, antibiotics, and DNA fragments in biodegradable polymeric particles. © 2007 Wiley Periodicals, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/bit.21334
dc.sourceScopus
dc.subjectCone-jet mode
dc.subjectElectrospray
dc.subjectMicroparticles
dc.subjectPLGA
dc.subjectProtein microencapsulation
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1002/bit.21334
dc.description.sourcetitleBiotechnology and Bioengineering
dc.description.volume97
dc.description.issue5
dc.description.page1278-1290
dc.description.codenBIBIA
dc.identifier.isiut000247960300025
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