Please use this identifier to cite or link to this item: https://doi.org/10.1002/aic.12525
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dc.titleControl and enhancement of permselectivity of membrane-based microcapsules for favorable biomolecular transport and immunoisolation
dc.contributor.authorRanganath, S.H.
dc.contributor.authorLing Tan, A.
dc.contributor.authorHe, F.
dc.contributor.authorWang, C.-H.
dc.contributor.authorKrantz, W.B.
dc.date.accessioned2014-10-09T06:45:15Z
dc.date.available2014-10-09T06:45:15Z
dc.date.issued2011-11
dc.identifier.citationRanganath, S.H., Ling Tan, A., He, F., Wang, C.-H., Krantz, W.B. (2011-11). Control and enhancement of permselectivity of membrane-based microcapsules for favorable biomolecular transport and immunoisolation. AIChE Journal 57 (11) : 3052-3062. ScholarBank@NUS Repository. https://doi.org/10.1002/aic.12525
dc.identifier.issn00011541
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/88695
dc.description.abstractThe success of membrane-based, cell-encapsulating microcapsules depends on the membrane permselectivity that provides efficient inward transport of nutrients, therapeutic protein egress, and complete exclusion of immunoglobulins. Microcapsules with a calcium crosslinked alginate core and a genipin-crosslinked chitosan alginate (GCA) were prepared with good control over size, membrane thickness and density. Importantly, in this study, we report a novel approach of using three relevant biomolecules and investigating the effects of the membrane characteristics (thickness and density) and microcapsule size on biomolecular mass transport across the GCA microcapsules using mathematical models based on a balance of the chemical potential. Scaling analysis was used to interrelate the membrane thickness, chitosan-alginate reaction rate constant, and diffusion coefficient. The resistance to diffusion of the three biomolecules increased with membrane density and thickness. Interestingly, swelling in the large microcapsules resulted in an increase in permeability, allowing larger biomolecules (immunoglobulin and carbonic anhydrase) to diffuse more readily. In the case of the smaller biomolecule, vitamin B12, a shorter diffusion path length in smaller microcapsules allowed better ingress. When compared with other microcapsules, the GCA microcapsules possess improved permselectivity for them to allow diffusion of small nutrient molecules and proteins, whereas completely excluding antibodies. Also, these results elucidate the importance of membrane properties and microcapsule size to realize favorable transport of biomolecules. © 2011 American Institute of Chemical Engineers (AIChE).
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/aic.12525
dc.sourceScopus
dc.subjectGenipin-chitosan membrane
dc.subjectMass transfer
dc.subjectMicrocapsules
dc.subjectPermeability
dc.subjectProtein delivery
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1002/aic.12525
dc.description.sourcetitleAIChE Journal
dc.description.volume57
dc.description.issue11
dc.description.page3052-3062
dc.description.codenAICEA
dc.identifier.isiut000296063500013
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