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|Title:||Conjugated Polymer Amplified Far-Red/Near-Infrared Fluorescence from Nanoparticles with Aggregation-Induced Emission Characteristics for Targeted In Vivo Imaging||Authors:||Ding, D.
Fluorescence resonance energy transfer
In vivo fluorescence imaging
|Issue Date:||Mar-2013||Citation:||Ding, D., Li, K., Qin, W., Zhan, R., Hu, Y., Liu, J., Tang, B.Z., Liu, B. (2013-03). Conjugated Polymer Amplified Far-Red/Near-Infrared Fluorescence from Nanoparticles with Aggregation-Induced Emission Characteristics for Targeted In Vivo Imaging. Advanced Healthcare Materials 2 (3) : 500-507. ScholarBank@NUS Repository. https://doi.org/10.1002/adhm.201200243||Abstract:||Fluorescence-amplified far-red/near-infrared (FR/NIR) nanoparticles (NPs) are synthesized by co-encapsulation of conjugated polymer donor (poly[9,9-bis(2-(2-(2-methoxyethoxy)ethoxy)ethyl)fluorenyldivinylene]; PFV) and a fluorogen acceptor (2-(2,6-bis((E)-4-(phenyl(4′-(1,2,2-triphenylvinyl)-[1,1′-biphenyl]-4-yl)amino)styryl)-4H-pyran-4-ylidene)malononitrile; TPE-TPA-DCM) with aggregation-induced emission (AIE) characteristics using biocompatible bovine serum albumin (BSA) as the encapsulation matrix. The good spectral overlap and close proximity between PFV and TPE-TPA-DCM in BSA NPs result in a 5.3-fold amplified TPE-TPA-DCM emission signal via fluorescence resonance energy transfer (FRET). The obtained PFV/TPE-TPA-DCM co-loaded BSA NPs are spherical in shape with a large Stokes shift of ∼223 nm and low cytotoxicity. The BSA matrix allows further functionalization with arginine-glycine-aspartic acid (RGD) peptide to yield fluorescent probes for specific recognition of integrin receptor-overexpressed cancer cells. The advantage of PFV amplified FR/NIR signal from TPE-TPA-DCM is further demonstrated in cellular and in vivo imaging using HT-29 colon cancer cells and a murine hepatoma H22 tumor-bearing mouse model, respectively. The high FR/NIR fluorescence and specific cancer targeting ability by RGD surface functionalization make the PFV/TPE-TPA-DCM co-loaded BSA-RGD NPs a unique FR/NIR fluorescent probe for cellular imaging and in vivo tumor diagnosis in a high contrast and selective manner. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.||Source Title:||Advanced Healthcare Materials||URI:||http://scholarbank.nus.edu.sg/handle/10635/88688||ISSN:||21922640||DOI:||10.1002/adhm.201200243|
|Appears in Collections:||Staff Publications|
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