Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.colsurfb.2003.08.007
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dc.titleConformational change of adsorbed and desorbed bovine serum albumin on nano-sized magnetic particles
dc.contributor.authorPeng, Z.G.
dc.contributor.authorHidajat, K.
dc.contributor.authorUddin, M.S.
dc.date.accessioned2014-10-09T06:45:08Z
dc.date.available2014-10-09T06:45:08Z
dc.date.issued2004-01-01
dc.identifier.citationPeng, Z.G., Hidajat, K., Uddin, M.S. (2004-01-01). Conformational change of adsorbed and desorbed bovine serum albumin on nano-sized magnetic particles. Colloids and Surfaces B: Biointerfaces 33 (1) : 15-21. ScholarBank@NUS Repository. https://doi.org/10.1016/j.colsurfb.2003.08.007
dc.identifier.issn09277765
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/88684
dc.description.abstractAdsorptions of bovine serum albumin (BSA) on nano-sized magnetic particles with and without the presence of carbodiimide were studied. Desorption of BSA from magnetic particles were carried out in either NaOH or Na 2HPO4 solutions. The structures of native BSA, adsorbed BSA on magnetic particles, and desorbed BSA were studied by several methods, circular dichroism (CD), fluorescence spectroscopy and differential scanning calorimetry (DSC). The magnitude of conformational changes of protein was determined by calculating the α-helix content from the circular dichroism (CD) spectra and by evaluating fluorescence spectrum and DSC thermograms. Adsorbed BSA on magnetic particles shows no thermal transition with respect to the native BSA. The structural change of BSA when desorbed by Na 2HPO4 solution is much smaller in comparison to that when desorbed by NaOH solution. Hence, this indicates that BSA could be desorbed from nano-sized magnetic particles using Na2HPO4 without much conformational change. © 2003 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.colsurfb.2003.08.007
dc.sourceScopus
dc.subjectAdsorption
dc.subjectBovine serum albumin (BSA)
dc.subjectConformational change
dc.subjectDesorption
dc.subjectNano-sized magnetic particles
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.colsurfb.2003.08.007
dc.description.sourcetitleColloids and Surfaces B: Biointerfaces
dc.description.volume33
dc.description.issue1
dc.description.page15-21
dc.description.codenCSBBE
dc.identifier.isiut000188512400003
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