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Title: Adsorption of bovine serum albumin on nanosized magnetic particles
Authors: Peng, Z.G.
Hidajat, K. 
Uddin, M.S. 
Keywords: Adsorption
Bovine serum albumin
Nanosized magnetic particles
Zeta potential
Issue Date: 15-Mar-2004
Citation: Peng, Z.G., Hidajat, K., Uddin, M.S. (2004-03-15). Adsorption of bovine serum albumin on nanosized magnetic particles. Journal of Colloid and Interface Science 271 (2) : 277-283. ScholarBank@NUS Repository.
Abstract: Adsorption of bovine serum albumin (BSA) on nanosized magnetic particles (Fe3O4) was carried out in the presence of carbodiimide. The equilibrium and kinetics of the adsorption process were studied. Nanosized magnetic particles (Fe3O4) were prepared by the chemical precipitation method using Fe2+, Fe3+ salts, and ammonium hydroxide under a nitrogen atmosphere. Characterizations of magnetic particles were carried out using transmission electron microscopy (TEM) and a vibrating sample magnetometer (VSM). Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used to confirm the attachment of BSA on magnetic particles. Effects of pH and salt concentrations were investigated on the adsorption process. The experimental results show that the adsorption of BSA on magnetic particles was affected greatly by the pH, while the effect of salt concentrations was insignificant at a low concentration range. The adsorption equilibrium isotherm was fitted well by the Langmuir model. The maximum adsorption of BSA on magnetic particles occurred at the isoelectric point of BSA. Adsorption kinetics was analyzed by a linear driving force mass-transfer model. BSA was desorbed from magnetic particles under alkaline conditions, which was confirmed by SDS-PAGE electrophoresis and FTIR results. © 2004 Published by Elsevier Inc.
Source Title: Journal of Colloid and Interface Science
ISSN: 00219797
DOI: 10.1016/j.jcis.2003.12.022
Appears in Collections:Staff Publications

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