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Title: Active hypothermic growth: A novel means for increasing total interferon-γ production by Chinese-hamster ovary cells
Authors: Fox, S.R.
Yap, M.X.
Yap, M.G.S. 
Wang, D.I.C.
Keywords: Active hypothermic growth
Controlled proliferation
Growth arrest
Low temperature
Issue Date: Jun-2005
Citation: Fox, S.R., Yap, M.X., Yap, M.G.S., Wang, D.I.C. (2005-06). Active hypothermic growth: A novel means for increasing total interferon-γ production by Chinese-hamster ovary cells. Biotechnology and Applied Biochemistry 41 (3) : 265-272. ScholarBank@NUS Repository.
Abstract: When grown under hypothermic conditions, CHO (Chinese-hamster ovary) cells become growth-arrested in the G0/G1 phase of the cell cycle and also often exhibit increased recombinant-protein production. We have shown in the accompanying paper [Fox, Tan, Tan, Wong, Yap and Wang (2005) Biotechnol. Appl. Biochem. 41, 255-264] that the positive effect of low temperature on recombinant-protein production is due to elevated mRNA levels and not due to G0/G1-phase growth arrest and that a cell line can still show growth-associated productivity at low temperature. This finding led to the hypothesis that improved total production of recombinant protein would be achieved by stimulating cells to actively grow at low temperature, a culture condition previously unreported in the literature. In the present study we have validated this hypothesis by stimulating hypothermic (32 °C) growth through the use of different growth factors. Hypothermic growth was stimulated in fetal-bovine-serum-supplemented adherent cultures using basic fibroblast growth factor or insulin. Hypothermic growth was also stimulated in suspension cultures normally grown in protein-free medium by using supplementation with fetal bovine serum. These methods resulted in up to 7.7- and 4.9-fold increases in total recombinant-protein production compared with the 37 and 32 °C control cultures respectively. This proof-of-concept study will motivate the creation of cell lines capable of growing at low temperatures for use in industrial processes. © 2005 Portland Press Ltd.
Source Title: Biotechnology and Applied Biochemistry
ISSN: 08854513
DOI: 10.1042/BA20040067
Appears in Collections:Staff Publications

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