Please use this identifier to cite or link to this item:
https://doi.org/10.1007/978-3-540-92841-6_207
DC Field | Value | |
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dc.title | Bead-based DNA Microarray Fabricated on Porous Polymer Films | |
dc.contributor.author | Cheng, J.T. | |
dc.contributor.author | Li, J. | |
dc.contributor.author | Chen, N.G. | |
dc.contributor.author | Gopalakrishnakone, P. | |
dc.contributor.author | Zhang, Y. | |
dc.date.accessioned | 2014-10-08T09:49:04Z | |
dc.date.available | 2014-10-08T09:49:04Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Cheng, J.T.,Li, J.,Chen, N.G.,Gopalakrishnakone, P.,Zhang, Y. (2009). Bead-based DNA Microarray Fabricated on Porous Polymer Films. IFMBE Proceedings 23 : 842-845. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-3-540-92841-6_207" target="_blank">https://doi.org/10.1007/978-3-540-92841-6_207</a> | |
dc.identifier.isbn | 9783540928409 | |
dc.identifier.issn | 16800737 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/88234 | |
dc.description.abstract | By attaching different biomolecule probes to surface-modified microspheres, a large number of complementary targets labeled with fluorophores can be interrogated simultaneously using the bead-based microarray format. In this work, a new bead-based DNA microarray was fabricated on a porous polymer film with well-ordered array of pores. The well-ordered porous polymer film was prepared using the non-lithographic breath figure method. Different biotinylated DNA probes were conjugated to avidin-modified polystyrene (PS) microspheres. The DNA microarray was fabricated by randomly dispersing DNA probe-conjugated PS microspheres into the pores on the polymer film. Multiplexing detection of DNA was performed using target DNAs labeled with ATTO dyes using a fluorescence microscope imaging system. The Bead-based DNA microarray was constructed for detection of four serotypes of Dengue virus. This technique was demonstrated to be a simple and cost-effective method for rapid detection of DNA targets. © International Federation of Medical and Biological Engineering 2009. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-3-540-92841-6_207 | |
dc.source | Scopus | |
dc.subject | DNA | |
dc.subject | Microarray | |
dc.subject | Microspheres | |
dc.subject | Pattern | |
dc.type | Conference Paper | |
dc.contributor.department | NUS NANOSCIENCE & NANOTECH INITIATIVE | |
dc.contributor.department | BIOENGINEERING | |
dc.description.doi | 10.1007/978-3-540-92841-6_207 | |
dc.description.sourcetitle | IFMBE Proceedings | |
dc.description.volume | 23 | |
dc.description.page | 842-845 | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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