Please use this identifier to cite or link to this item:
|Title:||Bead-based DNA Microarray Fabricated on Porous Polymer Films||Authors:||Cheng, J.T.
|Issue Date:||2009||Citation:||Cheng, J.T.,Li, J.,Chen, N.G.,Gopalakrishnakone, P.,Zhang, Y. (2009). Bead-based DNA Microarray Fabricated on Porous Polymer Films. IFMBE Proceedings 23 : 842-845. ScholarBank@NUS Repository. https://doi.org/10.1007/978-3-540-92841-6_207||Abstract:||By attaching different biomolecule probes to surface-modified microspheres, a large number of complementary targets labeled with fluorophores can be interrogated simultaneously using the bead-based microarray format. In this work, a new bead-based DNA microarray was fabricated on a porous polymer film with well-ordered array of pores. The well-ordered porous polymer film was prepared using the non-lithographic breath figure method. Different biotinylated DNA probes were conjugated to avidin-modified polystyrene (PS) microspheres. The DNA microarray was fabricated by randomly dispersing DNA probe-conjugated PS microspheres into the pores on the polymer film. Multiplexing detection of DNA was performed using target DNAs labeled with ATTO dyes using a fluorescence microscope imaging system. The Bead-based DNA microarray was constructed for detection of four serotypes of Dengue virus. This technique was demonstrated to be a simple and cost-effective method for rapid detection of DNA targets. © International Federation of Medical and Biological Engineering 2009.||Source Title:||IFMBE Proceedings||URI:||http://scholarbank.nus.edu.sg/handle/10635/88234||ISBN:||9783540928409||ISSN:||16800737||DOI:||10.1007/978-3-540-92841-6_207|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 11, 2019
checked on Oct 26, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.