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Title: Bead-based DNA Microarray Fabricated on Porous Polymer Films
Authors: Cheng, J.T. 
Li, J.
Chen, N.G. 
Gopalakrishnakone, P.
Zhang, Y. 
Keywords: DNA
Issue Date: 2009
Citation: Cheng, J.T.,Li, J.,Chen, N.G.,Gopalakrishnakone, P.,Zhang, Y. (2009). Bead-based DNA Microarray Fabricated on Porous Polymer Films. IFMBE Proceedings 23 : 842-845. ScholarBank@NUS Repository.
Abstract: By attaching different biomolecule probes to surface-modified microspheres, a large number of complementary targets labeled with fluorophores can be interrogated simultaneously using the bead-based microarray format. In this work, a new bead-based DNA microarray was fabricated on a porous polymer film with well-ordered array of pores. The well-ordered porous polymer film was prepared using the non-lithographic breath figure method. Different biotinylated DNA probes were conjugated to avidin-modified polystyrene (PS) microspheres. The DNA microarray was fabricated by randomly dispersing DNA probe-conjugated PS microspheres into the pores on the polymer film. Multiplexing detection of DNA was performed using target DNAs labeled with ATTO dyes using a fluorescence microscope imaging system. The Bead-based DNA microarray was constructed for detection of four serotypes of Dengue virus. This technique was demonstrated to be a simple and cost-effective method for rapid detection of DNA targets. © International Federation of Medical and Biological Engineering 2009.
Source Title: IFMBE Proceedings
ISBN: 9783540928409
ISSN: 16800737
DOI: 10.1007/978-3-540-92841-6_207
Appears in Collections:Staff Publications

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