Please use this identifier to cite or link to this item:
Title: The effect of micro and nanotopography on endocytosis in drug and gene delivery systems
Authors: Teo, B.K.K.
Goh, S.-H.
Kustandi, T.S.
Loh, W.W.
Low, H.Y.
Yim, E.K.F. 
Keywords: Human mesenchymal stem cells
Non-viral transfection
Transfection efficiency
Issue Date: Dec-2011
Citation: Teo, B.K.K., Goh, S.-H., Kustandi, T.S., Loh, W.W., Low, H.Y., Yim, E.K.F. (2011-12). The effect of micro and nanotopography on endocytosis in drug and gene delivery systems. Biomaterials 32 (36) : 9866-9875. ScholarBank@NUS Repository.
Abstract: Endocytosis is a fundamental biological process and is also the key mechanism for drug and non-viral gene delivery. The importance of topographical cues in modulating cell behaviors has become increasingly evident, but the influence of topography on endocytosis has however only been sparsely studied. We hypothesize that topography can enhance cellular endocytosis, and in turn the non-viral transfection efficiency. Nano- to microtopographical patterns were fabricated using nano-imprinting lithography (NIL). We first investigated if the substrate topographies could modulate endocytosis and in turn the cellular transfectability. Our results showed increased internalization of fluorescently labeled dextran by human mesenchymal stem cells (hMSCs) and monkey kidney fibroblasts (COS7) when they were cultured on micro- and nanopillars. When the hMSCs were introduced to green-fluorescent protein (GFP) encoding plasmid with Lipofectamine, highest transfection efficiency was observed in cells on nanopillars. Tunable detachable substrate topographies were also fabricated using NIL to promote endocytosis in different cell types, and our results show hMSCs phagocytosis of these polymeric structures. Besides being important in understanding the fundamental process of endocytosis, the current research results may also lead to applications utilizing nanotopography to enhance drug and gene delivery. © 2011 Elsevier Ltd.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2011.08.088
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jul 1, 2020


checked on Jun 24, 2020

Page view(s)

checked on Jun 28, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.