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Title: High-throughput cell cycle synchronization using inertial forces in spiral microchannels
Authors: Lee, W.C.
Bhagat, A.A.S.
Huang, S.
Van Vliet, K.J.
Han, J.
Lim, C.T. 
Issue Date: 7-Apr-2011
Citation: Lee, W.C., Bhagat, A.A.S., Huang, S., Van Vliet, K.J., Han, J., Lim, C.T. (2011-04-07). High-throughput cell cycle synchronization using inertial forces in spiral microchannels. Lab on a Chip - Miniaturisation for Chemistry and Biology 11 (7) : 1359-1367. ScholarBank@NUS Repository.
Abstract: Efficient synchronization and selection of cells at different stages of the cell replication cycle facilitates both fundamental research and development of cell cycle-targeted therapies. Current chemical-based synchronization methods are unfavorable as these can disrupt cell physiology and metabolism. Microfluidic systems developed for physical cell separation offer a potential alternative over conventional cell synchronization approaches. Here we introduce a spiral microfluidic device for cell cycle synchronization, using the combined effects of inertial forces and Dean drag force. By exploiting the relationship between cell diameter and cell cycle (DNA content/ploidy), we have successfully fractionated several asynchronous mammalian cell lines, as well as primary cells comprising bone marrow-derived human mesenchymal stem cells (hMSCs), into enriched subpopulations of G0/G1 (>85%), S, and G2/M phases. This level of cell cycle enrichment is comparable to existing microfluidic systems, but the throughput (∼15 × 106 cells per h) and viability (∼95%) of cells thus synchronized are significantly greater. Further, this platform provides rapid collection of synchronized cells or of diameter-sorted cells post-separation, to enable diverse applications in the study and manipulation of cell proliferation. © The Royal Society of Chemistry.
Source Title: Lab on a Chip - Miniaturisation for Chemistry and Biology
ISSN: 14730197
DOI: 10.1039/c0lc00579g
Appears in Collections:Staff Publications

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