Please use this identifier to cite or link to this item: https://doi.org/10.1021/ac301119r
DC FieldValue
dc.titleHigh-performance graphene-titania platform for detection of phosphopeptides in cancer cells
dc.contributor.authorTang, L.A.L.
dc.contributor.authorWang, J.
dc.contributor.authorLim, T.K.
dc.contributor.authorBi, X.
dc.contributor.authorLee, W.C.
dc.contributor.authorLin, Q.
dc.contributor.authorChang, Y.-T.
dc.contributor.authorLim, C.T.
dc.contributor.authorLoh, K.P.
dc.date.accessioned2014-10-08T09:44:11Z
dc.date.available2014-10-08T09:44:11Z
dc.date.issued2012-08-07
dc.identifier.citationTang, L.A.L., Wang, J., Lim, T.K., Bi, X., Lee, W.C., Lin, Q., Chang, Y.-T., Lim, C.T., Loh, K.P. (2012-08-07). High-performance graphene-titania platform for detection of phosphopeptides in cancer cells. Analytical Chemistry 84 (15) : 6693-6700. ScholarBank@NUS Repository. https://doi.org/10.1021/ac301119r
dc.identifier.issn00032700
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/87817
dc.description.abstractPhosphopeptides play a crucial role in many biological processes and constitute some of the most powerful biomarkers in disease detection. However they are often present in very low concentration, which makes their detection highly challenging. Here, we demonstrate the use of a solution-dispersible graphene-titania platform for the selective extraction of phosphopeptides from peptide mixtures. This is followed by direct analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The efficient charge and energy exchange between graphene and TiO2 during laser irradiation in SELDI-TOF MS promotes the soft ionization of analytes and affords a detection limit in the attomole range, which is 102-10 5 more sensitive than conventional platforms. The graphene-titania platform can also be used for detecting phosphopeptides in cancer cells (HeLa cells), where it shows high specificity (94%). An expanded library of 967 unique phosphopeptides is detected using significantly reduced loading of extraction matrixes compared to conventional TiO2 bead-based assays. © 2012 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/ac301119r
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1021/ac301119r
dc.description.sourcetitleAnalytical Chemistry
dc.description.volume84
dc.description.issue15
dc.description.page6693-6700
dc.description.codenANCHA
dc.identifier.isiut000307159200061
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

56
checked on Nov 23, 2020

WEB OF SCIENCETM
Citations

52
checked on Nov 23, 2020

Page view(s)

83
checked on Nov 22, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.