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Title: Electrochemical bioassay utilizing encapsulated electrochemical active microcrystal biolabels
Authors: Wing, C.M.
Kwan, Y.C.
Trau, D. 
Warsinke, A.
Scheller, F.
Renneberg, R.
Issue Date: 1-May-2005
Citation: Wing, C.M., Kwan, Y.C., Trau, D., Warsinke, A., Scheller, F., Renneberg, R. (2005-05-01). Electrochemical bioassay utilizing encapsulated electrochemical active microcrystal biolabels. Analytical Chemistry 77 (9) : 2835-2841. ScholarBank@NUS Repository.
Abstract: A new approach to perform electrochemical immunoassay based on the utilization of encapsulated microcrystal was developed. The microcrystal labels create a "supernova effect" upon exposure to a desired releasing agent. The microcrystal cores dissolve, and large amounts of signal-generating molecules diffuse across the capsule wall into the outer environment. Layer-by-Layer (LbL) technology was employed for the encapsulation of electrochemical signal-generating microcrystals (ferrocene microcrystals). The encapsulated microcrystals were conjugated with antibody molecules through the adsorption process. The biofunctionalized microcrystals were utilized as a probe for immunoassays. The microcrystal-based label system provided a high-signal molecule to antibody (S/P) ratio of 104-105. Microcrystal biolabels with different antibody surface coverage (1.60-5.05 mg m-2) were subjected to a solid-phase immunoassay for the detection of mouse immunoglobulin G (M-IgG) molecules. The microcrystal-based immunoassay for the detection of M-IgG performed with microcrystals having antibody surface coverage of 5.05 mg m-2 showed a sensitivity of 3.93 nA μg-1 L-1 with a detection limit of 2.82 μg L-1. © 2005 American Chemical Society.
Source Title: Analytical Chemistry
ISSN: 00032700
DOI: 10.1021/ac048505l
Appears in Collections:Staff Publications

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