Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2013.12.060
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dc.titleBacterial imaging with photostable upconversion fluorescent nanoparticles
dc.contributor.authorOng, L.C.
dc.contributor.authorAng, L.Y.
dc.contributor.authorAlonso, S.
dc.contributor.authorZhang, Y.
dc.date.accessioned2014-10-08T09:43:02Z
dc.date.available2014-10-08T09:43:02Z
dc.date.issued2014-03
dc.identifier.citationOng, L.C., Ang, L.Y., Alonso, S., Zhang, Y. (2014-03). Bacterial imaging with photostable upconversion fluorescent nanoparticles. Biomaterials 35 (9) : 2987-2998. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2013.12.060
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/87717
dc.description.abstractAutofluorescence, photodamage and photobleaching are often encountered when using downconverting fluorophores and fluorescent proteins for bacteria labeling. These caveats represent a serious limitation when trying to map bacteria dissemination for prolonged periods. Upconversion nanoparticles (UCNs), which are able to convert low energy near-infrared (NIR) excitation light into higher energy visible or NIR light, can address these limitations. These particles' unique optical properties translate into attractive advantages of minimal autofluorescence, reduced photodamage, deeper tissue penetration and prolonged photostability. Here, we report a UCN-based bacteria labeling strategy using Escherichia coli as prototypic bacteria. A comparative analysis highlighted the superior photostability of UCN-labeled bacteria over green fluorescent protein-expressing bacteria. Infection study of UCN-labeled bacteria in dendritic cells indicated co-localization of the UCN signal with bacterial position for up to 6h post-infection. Furthermore, long-term monitoring of the same infected cells demonstrated the potential to utilize photostable UCN-based imaging for bacterial trafficking purposes. © 2013 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2013.12.060
dc.sourceScopus
dc.subjectBacteria labeling
dc.subjectEscherichia coli
dc.subjectFluorescence imaging
dc.subjectUpconversion nanoparticles
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1016/j.biomaterials.2013.12.060
dc.description.sourcetitleBiomaterials
dc.description.volume35
dc.description.issue9
dc.description.page2987-2998
dc.description.codenBIMAD
dc.identifier.isiut000332188900042
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