Please use this identifier to cite or link to this item: https://doi.org/10.1117/1.3655353
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dc.titleAssessment of liver steatosis and fibrosis in rats using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique
dc.contributor.authorLin, J.
dc.contributor.authorLu, F.
dc.contributor.authorZheng, W.
dc.contributor.authorXu, S.
dc.contributor.authorTai, D.
dc.contributor.authorYu, H.
dc.contributor.authorHuang, Z.
dc.date.accessioned2014-10-08T09:42:59Z
dc.date.available2014-10-08T09:42:59Z
dc.date.issued2011-11
dc.identifier.citationLin, J., Lu, F., Zheng, W., Xu, S., Tai, D., Yu, H., Huang, Z. (2011-11). Assessment of liver steatosis and fibrosis in rats using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique. Journal of Biomedical Optics 16 (11) : -. ScholarBank@NUS Repository. https://doi.org/10.1117/1.3655353
dc.identifier.issn10833668
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/87714
dc.description.abstractWe report the implementation of a unique integrated coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and two-photon excitation fluorescence (TPEF) microscopy imaging technique developed for label-free monitoring of the progression of liver steatosis and fibrosis generated in a bile duct ligation (BDL) rat model. Among the 21 adult rats used in this study, 18 rats were performed with BDL surgery and sacrificed each week from weeks 1 to 6 (n = 3 per week), respectively; whereas 3 rats as control were sacrificed at week 0. Colocalized imaging of the aggregated hepatic fats, collagen fibrils, and hepatocyte morphologies in liver tissue is realized by using the integrated CARS, SHG, and TPEF technique. The results show that there are significant accumulations of hepatic lipid droplets and collagen fibrils associated with severe hepatocyte necrosis in BDL rat liver as compared to a normal liver tissue. The volume of normal hepatocytes keeps decreasing and the fiber collagen content in BDL rat liver follows a growing trend until week 6; whereas the hepatic fat content reaches a maximum in week 4 and then appears to stop growing in week 6, indicating that liver steatosis and fibrosis induced in a BDL rat liver model may develop at different rates. This work demonstrates that the integrated CARS and multiphoton microscopy imaging technique has the potential to provide an effective means for early diagnosis and detection of liver steatosis and fibrosis without labeling. © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1117/1.3655353
dc.sourceScopus
dc.subjectBile duct ligation
dc.subjectCoherent anti-Stokes Raman scattering
dc.subjectCollagen fibrils
dc.subjectFibrosis
dc.subjectHepatic fat
dc.subjectLiver
dc.subjectMicroscopy
dc.subjectSecond harmonic generation
dc.subjectSteatosis
dc.subjectTwo-photon excitation fluorescence
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.description.doi10.1117/1.3655353
dc.description.sourcetitleJournal of Biomedical Optics
dc.description.volume16
dc.description.issue11
dc.description.page-
dc.description.codenJBOPF
dc.identifier.isiut000298546500031
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