Please use this identifier to cite or link to this item:
|Title:||Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanism||Authors:||Lin, W.
CHOO BOON HWA,ANDRE
|Keywords:||Embryonic stem cells
Mesenchymal stromal cells
|Issue Date:||Mar-2012||Citation:||Lin, W., Oh, S.K.W., CHOO BOON HWA,ANDRE, George, A.J.T. (2012-03). Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanism. Cytotherapy 14 (3) : 274-284. ScholarBank@NUS Repository. https://doi.org/10.3109/14653249.2011.635853||Abstract:||Background aims. Human embryonic stem cell (hESC)-derived mesenchymal stromal cells (MSC) (hESC-MSC) are an alternative source of MSC to bone marrow (BM)-derived MSC (BM-MSC), which are being investigated in clinical trials for their immunomodulatory potential. hESC-MSC have the advantage of being consistent because each batch can be generated from hESC under defined conditions. In contrast, BM-MSC have a limited proliferative capacity. Methods. The ability to suppress the proliferation of anti-CD3/CD28-stimulated CD4 + T cells by hESC-MSC was compared with adult BM-MSC and neonatal foreskin fibroblast (Fb). Results. hESC-MSC suppress the proliferation of CD4 + T cells in both contact and transwell systems, although inhibition is less in the transwell system. hESC-MSC are approximately 2-fold less potent (67 cells/100 T cells) than BM-MSC and Fb (37 and 34 cells/100 T cells, respectively) at suppressing T-cell proliferation by 50% in a transwell [inhibitory concentration(IC)50]. The anti-proliferative effect is not contact-dependent but requires the presence of factors such as interferon (IFN)-γ produced by activated T cells. IFN-γ induces the expression of indoleamine-2,3-dioxygenase (IDO) in hESC-MSC, BM-MSC and Fb, contributing to their immunosuppressive property. Conclusions. The feedback loop between MSC or Fb and activated T cells may limit the immunosuppressive effects of MSC and Fb to sites containing ongoing immunologic or inflammatory responses where activated T cells induce the up-regulation of IDO and immunomodulatory properties of MSC and Fb. These data demonstrate that hESC-MSC may be evaluated further as an allogeneic cell source for therapeutic applications requiring immunosuppression. © 2012 Informa Healthcare.||Source Title:||Cytotherapy||URI:||http://scholarbank.nus.edu.sg/handle/10635/87702||ISSN:||14653249||DOI:||10.3109/14653249.2011.635853|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 24, 2021
WEB OF SCIENCETM
checked on Jan 15, 2021
checked on Jan 18, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.