Please use this identifier to cite or link to this item: https://doi.org/10.1109/MMB.2006.251549
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dc.titlePolyacrylamide micro-pillar based DNA microarray and its application for genetic variation analysis
dc.contributor.authorSelamat, E.S.
dc.contributor.authorHong, P.Y.
dc.contributor.authorLiu, W.-T.
dc.date.accessioned2014-10-08T08:34:15Z
dc.date.available2014-10-08T08:34:15Z
dc.date.issued2006
dc.identifier.citationSelamat, E.S., Hong, P.Y., Liu, W.-T. (2006). Polyacrylamide micro-pillar based DNA microarray and its application for genetic variation analysis. Proceedings of 2006 International Conference on Microtechnologies in Medicine and Biology : 277-279. ScholarBank@NUS Repository. https://doi.org/10.1109/MMB.2006.251549
dc.identifier.isbn1424403383
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/87675
dc.description.abstractTo improve diffusive capabilities and target accessibility with a gel-based microarray, a novel 'pads within a pad' approach was developed. Using a soft lithography technique, an array of 'waffle'-like micro-pillars embedded on the surface of the glass slide substratum was fabricated. The micro-pillar based microarray showed more than a 3-fold increase in the effective surface area available for probe immobilization. As a result, a 5-fold increase was observed in the hybridization rate and intensity of the 'waffle' pad as compared to an unmodified gel pad. The functionality of the 'waffle' pad was demonstrated by the accurate genotyping of two sets of SNPs (SNP_1236 and SNP_2677) based on the dissociation curve, dissociation temperature, Td and the difference of Td between the perfect match duplex with that of the mismatch (ΔT d). ©2006 IEEE.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1109/MMB.2006.251549
dc.sourceScopus
dc.subject'Pads within a pad'
dc.subjectDissociation temperature
dc.subjectMicro-pillars
dc.subjectPolyacrylamide gel
dc.typeConference Paper
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.description.doi10.1109/MMB.2006.251549
dc.description.sourcetitleProceedings of 2006 International Conference on Microtechnologies in Medicine and Biology
dc.description.page277-279
dc.identifier.isiut000244177200083
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