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Title: Rapid discrimination of single-nucleotide mismatches using a microfluidic device with monolayered beads
Authors: Ng, J.K.-K.
Feng, H.
Liu, W.-T. 
Keywords: Dissociation kinetics
Microfluidic device
Single-nucleotide mismatches
Single-nucleotide polymorphisms (SNPs)
Issue Date: 23-Jan-2007
Citation: Ng, J.K.-K., Feng, H., Liu, W.-T. (2007-01-23). Rapid discrimination of single-nucleotide mismatches using a microfluidic device with monolayered beads. Analytica Chimica Acta 582 (2) : 295-303. ScholarBank@NUS Repository.
Abstract: A microfluidic device incorporating monolayered beads is developed for the discrimination of single-nucleotide mismatches, based on the differential dissociation kinetics between perfect match (PM) and mismatched (MM) duplexes. The monolayered beads are used as solid support for the immobilization of oligonucleotide probes containing a single-base variation. Target oligonucleotides hybridize to the probes, forming either PM duplexes or MM duplexes containing a single mismatch. Optimization studies show that PM and MM duplexes are easily discriminated based on their dissociation but not hybridization kinetics under an optimized buffer composition of 100 mM NaCl and 50% formamide. Detection of single-nucleotide polymorphism (SNP) using the device is demonstrated within 8 min using four probes containing all the possible single-base variants. The device can easily be modified to integrate multiplexed detection, making high-throughput SNP detection possible. © 2006 Elsevier B.V. All rights reserved.
Source Title: Analytica Chimica Acta
ISSN: 00032670
DOI: 10.1016/j.aca.2006.09.016
Appears in Collections:Staff Publications

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