Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/87553
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dc.titleMicrobial detection in microfluidic devices through dual staining of quantum dots-labeled immunoassay and RNA hybridization
dc.contributor.authorZhang, Q.
dc.contributor.authorZhu, L.
dc.contributor.authorFeng, H.
dc.contributor.authorAng, S.
dc.contributor.authorChau, F.S.
dc.contributor.authorLiu, W.-T.
dc.date.accessioned2014-10-08T08:32:52Z
dc.date.available2014-10-08T08:32:52Z
dc.date.issued2006-01-18
dc.identifier.citationZhang, Q., Zhu, L., Feng, H., Ang, S., Chau, F.S., Liu, W.-T. (2006-01-18). Microbial detection in microfluidic devices through dual staining of quantum dots-labeled immunoassay and RNA hybridization. Analytica Chimica Acta 556 (1) : 171-177. ScholarBank@NUS Repository.
dc.identifier.issn00032670
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/87553
dc.description.abstractThis paper reported the development of a microfludic device for the rapid detection of viable and nonviable microbial cells through dual labeling by fluorescent in situ hybridization (FISH) and quantum dots (QDs)-labeled immunofluorescent assay (IFA). The coin sized device consists of a microchannel and filtering pillars (gap = 1-2 μm) and was demonstrated to effectively trap and concentrate microbial cells (i.e. Giardia lamblia). After sample injection, FISH probe solution and QDs-labeled antibody solution were sequentially pumped into the device to accelerate the fluorescent labeling reactions at optimized flow rates (i.e. 1 and 20 μL/min, respectively). After 2 min washing for each assay, the whole process could be finished within 30 min, with minimum consumption of labeling reagents and superior fluorescent signal intensity. The choice of QDs 525 for IFA resulted in bright and stable fluorescent signal, with minimum interference with the Cy3 signal from FISH detection. © 2005 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.aca.2005.07.003
dc.sourceScopus
dc.subjectFISH
dc.subjectGiardia lamblia
dc.subjectImmunofluorescent assay
dc.subjectMicrofluidic device
dc.subjectQuantum dots
dc.typeArticle
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.contributor.departmentMECHANICAL ENGINEERING
dc.contributor.departmentCIVIL ENGINEERING
dc.description.sourcetitleAnalytica Chimica Acta
dc.description.volume556
dc.description.issue1
dc.description.page171-177
dc.description.codenACACA
dc.identifier.isiut000234724900021
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