Please use this identifier to cite or link to this item: https://doi.org/10.1128/AEM.00185-10
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dc.titleEffect of host cells on low- and medium-pressure UV inactivation of adenoviruses
dc.contributor.authorGuo, H.
dc.contributor.authorChu, X.
dc.contributor.authorHu, J.
dc.date.accessioned2014-10-08T08:32:08Z
dc.date.available2014-10-08T08:32:08Z
dc.date.issued2010-11
dc.identifier.citationGuo, H., Chu, X., Hu, J. (2010-11). Effect of host cells on low- and medium-pressure UV inactivation of adenoviruses. Applied and Environmental Microbiology 76 (21) : 7068-7075. ScholarBank@NUS Repository. https://doi.org/10.1128/AEM.00185-10
dc.identifier.issn00992240
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/87490
dc.description.abstractUV disinfection is highly effective against most pathogens, with the exception of the adenoviruses (AD). To date, many studies have focused on low-pressure (LP) UV inactivation of AD, but little is known about the effect of medium-pressure (MP) UV inactivation of AD. Despite numerous studies of LP UV inactivation of AD, extreme variabilities in the LP UV dose requirements of AD had been observed because of differing experimental conditions used, such as the types of cell lines used for AD enumeration. This study therefore investigates the effect of three different host cell lines (PLC/PRF/5, human embryonic kidney 293 [HEK293], and XP17BE) on the LP and MP UV dose requirements of AD serotype 5 (AD5), AD40, and AD41 under similar experimental settings. Results showed that for 4-log inactivation of AD, LP UV and MP UV doses needed to be in the ranges of 123 to 182 mJ/cm2 and 65 to 90 mJ/cm2, respectively, when HEK293 and PLC/PRF/5 cells were used for enumeration. The UV doses required for MP UV inactivation of AD were significantly lower than those required for LP UV inactivation (P value < 0.05). When different cell lines were used for enumeration, UV dose requirements for AD differed. AD were portrayed to be most susceptible to UV (LP UV doses of
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/AEM.00185-10
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.description.doi10.1128/AEM.00185-10
dc.description.sourcetitleApplied and Environmental Microbiology
dc.description.volume76
dc.description.issue21
dc.description.page7068-7075
dc.description.codenAEMID
dc.identifier.isiut000283439800012
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