Please use this identifier to cite or link to this item:
Title: The challenge to measure cell proliferation in two and three dimensions
Authors: Ng, K.W. 
Leong, D.T.W. 
Hutmacher, D.W. 
Issue Date: Jan-2005
Citation: Ng, K.W., Leong, D.T.W., Hutmacher, D.W. (2005-01). The challenge to measure cell proliferation in two and three dimensions. Tissue Engineering 11 (1-2) : 182-191. ScholarBank@NUS Repository.
Abstract: Various assays, using different strategies, are available for assessing cultured cell proliferation. These include measurement of metabolic activity (tetrazolium salts and alamarBlue), DNA quantification using fluorophores (Hoechst 33258 and PicoGreen), uptake of radioactively-labeled DNA precursors such as [3H]thymidine, and physical counting (hemocytometer). These assays are well established in characterizing cell proliferation in two-dimensional (2D), monolayer cultures of low cell densities. However, increasing interest in 3D cultures has prompted the need to evaluate the effectiveness of using these assays in high cell density or 3D cultures. We show here that typical cell proliferation assays do not necessarily correlate linearly with increasing cell densities or between 2D and 3D cultures, and are either not suitable or only rough approximations in quantifying actual cell numbers in a culture. Prudent choice of techniques and careful interpretation of data are therefore recommended when measuring cell proliferation in high cell density and 3D cultures.
Source Title: Tissue Engineering
ISSN: 10763279
DOI: 10.1089/ten.2005.11.182
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 25, 2021


checked on Feb 18, 2021

Page view(s)

checked on Feb 15, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.