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https://doi.org/10.1016/j.biomaterials.2006.06.017
| Title: | Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells | Authors: | Chua, K.-N. Chai, C. Lee, P.-C. Tang, Y.-N. Ramakrishna, S. Leong, K.W. Mao, H.-Q. |
Keywords: | Electrospinning Expansion Hematopoietic stem cells Nanofiber Stem cell therapy |
Issue Date: | Dec-2006 | Citation: | Chua, K.-N., Chai, C., Lee, P.-C., Tang, Y.-N., Ramakrishna, S., Leong, K.W., Mao, H.-Q. (2006-12). Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells. Biomaterials 27 (36) : 6043-6051. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2006.06.017 | Abstract: | Interaction between hematopoietic stem/progenitor cells (HSPCs) and their extra cellular matrix components is an integral part of the signaling control for HSPC survival, proliferation and differentiation. We hypothesized that both substrate topographical cues and biochemical cues could act synergistically with cytokine supplementation to improve ex vivo expansion of HSPCs. In this study, we compared the ex vivo expansion of human umbilical cord blood CD34+ cells on unmodified, hydroxylated, carboxylated and aminated nanofibers and films. Results from 10-day expansion cultures showed that aminated nanofiber mesh and film were most efficient in supporting the expansion of the CD34+CD45+ cells (195-fold and 178-fold, respectively), as compared to tissue culture polystyrene (50-fold, p | Source Title: | Biomaterials | URI: | http://scholarbank.nus.edu.sg/handle/10635/85711 | ISSN: | 01429612 | DOI: | 10.1016/j.biomaterials.2006.06.017 |
| Appears in Collections: | Staff Publications |
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