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Title: NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA
Authors: Jiang, S.
Zhang, Y. 
Lim, K.M. 
Sim, E.K.W.
Ye, L.
Issue Date: 2009
Citation: Jiang, S., Zhang, Y., Lim, K.M., Sim, E.K.W., Ye, L. (2009). NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA. Nanotechnology 20 (15) : -. ScholarBank@NUS Repository.
Abstract: Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules. © 2009 IOP Publishing Ltd.
Source Title: Nanotechnology
ISSN: 09574484
DOI: 10.1088/0957-4484/20/15/155101
Appears in Collections:Staff Publications

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