Please use this identifier to cite or link to this item:
|Title:||NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA||Authors:||Jiang, S.
|Issue Date:||2009||Citation:||Jiang, S., Zhang, Y., Lim, K.M., Sim, E.K.W., Ye, L. (2009). NIR-to-visible upconversion nanoparticles for fluorescent labeling and targeted delivery of siRNA. Nanotechnology 20 (15) : -. ScholarBank@NUS Repository. https://doi.org/10.1088/0957-4484/20/15/155101||Abstract:||Near-infrared (NIR)-to-visible upconversion fluorescent nanoparticles were synthesized and used for imaging and targeted delivery of small interfering RNA (siRNA) to cancer cells. Silica-coated NaYF4 upconversion nanoparticles (UCNs) co-doped with lanthanide ions (Yb/Er) were synthesized. Folic acid and anti-Her2 antibody conjugated UCNs were used to fluorescently label the folate receptors of HT-29 cells and Her2 receptors of SK-BR-3 cells, respectively. The intracellular uptake of the folic acid and antibody conjugated UCNs was visualized using a confocal fluorescence microscope equipped with an NIR laser. siRNA was attached to anti-Her2 antibody conjugated UCNs and the delivery of these nanoparticles to SK-BR-3 cells was studied. Meanwhile, a luciferase assay was established to confirm the gene silencing effect of siRNA. Upconversion nanoparticles can serve as a fluorescent probe and delivery system for simultaneous imaging and delivery of biological molecules. © 2009 IOP Publishing Ltd.||Source Title:||Nanotechnology||URI:||http://scholarbank.nus.edu.sg/handle/10635/85483||ISSN:||09574484||DOI:||10.1088/0957-4484/20/15/155101|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 15, 2020
WEB OF SCIENCETM
checked on Sep 8, 2020
checked on Sep 13, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.