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|Title:||A comparative molecular force spectroscopy study of homophilic JAM-A interactions and JAM-A interactions with reovirus attachment protein σ1||Authors:||Vedula, S.R.K.
|Keywords:||Atomic force microscopy (AFM)
Junctional adhesion molecule-A (JAM-A)
Molecular force spectroscopy
Reovirus attachment protein (sigma1)
|Issue Date:||Jul-2008||Citation:||Vedula, S.R.K., Lim, T.S., Kirchner, E., Guglielmi, K.M., Dermody, T.S., Stehle, T., Hunziker, W., Lim, C.T. (2008-07). A comparative molecular force spectroscopy study of homophilic JAM-A interactions and JAM-A interactions with reovirus attachment protein σ1. Journal of Molecular Recognition 21 (4) : 210-216. ScholarBank@NUS Repository. https://doi.org/10.1002/jmr.886||Abstract:||JAM-A belongs to a family of immunoglobulin-like proteins called junctional adhesion molecules (JAMs) that localize at epithelial and endothelial intercellular tight junctions. JAM-A is also expressed on dendritic cells, neutrophils, and platelets. Homophilic JAM-A interactions play an important role in regulating paracellular permeability and leukocyte transmigration across epithelial monolayers and endothelial cell junctions, respectively. In addition, JAM-A is a receptor for the reovirus attachment protein, σ1. In this study, we used single molecular force spectroscopy to compare the kinetics of JAM-A interactions with itself and σ1. A chimeric murine JAM-A/Fc fusion protein and the purified σ1 head domain were used to probe murine L929 cells, which express JAM-A and are susceptible to reovirus infection. The bond half-life (t1/2) of homophilic JAM-A interactions was found to be shorter (koff o = 0.688 ± 0.349 s-1) than that of σ1/JAM-A interactions (koff o = 0.067 ± 0.041 s-1). These results are in accordance with the physiological functions of JAM-A and σ1. A short bond lifetime imparts a highly dynamic nature to homophilic JAM-A interactions for regulating tight junction permeability while stable interactions between σ1 and JAM-A likely anchor the virus to the cell surface and facilitate viral entry. Copyright © 2008 John Wiley & Sons, Ltd.||Source Title:||Journal of Molecular Recognition||URI:||http://scholarbank.nus.edu.sg/handle/10635/84770||ISSN:||09523499||DOI:||10.1002/jmr.886|
|Appears in Collections:||Staff Publications|
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