Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0141-0229(02)00287-9
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dc.titleOptimization of agitation, aeration, and temperature conditions for maximum β-mannanase production
dc.contributor.authorFeng, Y.
dc.contributor.authorHe, Z.
dc.contributor.authorOng, S.L.
dc.contributor.authorHu, J.
dc.contributor.authorZhang, Z.
dc.contributor.authorNg, W.J.
dc.date.accessioned2014-10-07T06:27:33Z
dc.date.available2014-10-07T06:27:33Z
dc.date.issued2003-02-03
dc.identifier.citationFeng, Y., He, Z., Ong, S.L., Hu, J., Zhang, Z., Ng, W.J. (2003-02-03). Optimization of agitation, aeration, and temperature conditions for maximum β-mannanase production. Enzyme and Microbial Technology 32 (2) : 282-289. ScholarBank@NUS Repository. https://doi.org/10.1016/S0141-0229(02)00287-9
dc.identifier.issn01410229
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/84645
dc.description.abstractThe effects of cultivation temperature, aeration rate, and agitation speed on the production of β-mannanase by Bacillus licheniformis NK-27 in a batch fermenter were investigated in this study. Results revealed that temperature was the most significant factor in terms of its effect on β-mannanase production. It influenced β-mannanase production by affecting the other parameters including bacteria growth, pH, dissolved oxygen, total and reducing sugars. Agitation speed and aeration rate could affect dissolved oxygen concentration which in turn affected cell growth and β-mannanase production. A maximum β-mannanase activity of 212.0Uml-1 was attained in 36h of cultivation when aeration rate, agitation speed, and temperature were controlled at 0.75vvm, 600rpm, and 30°C, respectively. The maximum β-mannanase activity in the fermenter was close to that obtained from the shake flask fermentation study (198.2Uml-1). However, the duration of fermentation cycle in the fermenter study was shorter than the corresponding duration obtained from the shake flask experiment by 12h. © 2002 Elsevier Science Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0141-0229(02)00287-9
dc.sourceScopus
dc.subjectβ-Mannanase
dc.subjectAeration rate
dc.subjectAgitation speed
dc.subjectBacillus licheniformis
dc.subjectTemperature
dc.typeArticle
dc.contributor.departmentCIVIL ENGINEERING
dc.description.doi10.1016/S0141-0229(02)00287-9
dc.description.sourcetitleEnzyme and Microbial Technology
dc.description.volume32
dc.description.issue2
dc.description.page282-289
dc.description.codenEMTED
dc.identifier.isiut000180692300010
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