Please use this identifier to cite or link to this item: https://doi.org/10.1116/1.3049529
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dc.titleDevelopment of microfluidic device and system for breast cancer cell fluorescence detection
dc.contributor.authorHe, J.H.
dc.contributor.authorReboud, J.
dc.contributor.authorJi, H.M.
dc.contributor.authorLee, C.
dc.contributor.authorLong, Y.
dc.date.accessioned2014-10-07T04:43:22Z
dc.date.available2014-10-07T04:43:22Z
dc.date.issued2009
dc.identifier.citationHe, J.H., Reboud, J., Ji, H.M., Lee, C., Long, Y. (2009). Development of microfluidic device and system for breast cancer cell fluorescence detection. Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures 27 (3) : 1295-1298. ScholarBank@NUS Repository. https://doi.org/10.1116/1.3049529
dc.identifier.issn10711023
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/83627
dc.description.abstractA biomicrofluidic device and a compact cellular testing system were developed to be used in cancer diagnostics. The device was fabricated by lithography-based microfabrication techniques, followed by two-step etching of deep reactive ion etching, and channels were formed by anodic bonding of Si and Pyrex. The device is based on the capture of cells inside a new meandering weir-type filter design, followed by detection and characterization using specific fluorescent labeling. Breast cancer cells MCF-7 and control cells MCF-10A were flowed through the microfluidic channels, and captured by meandering weir-type filters. 17Β-Estradiaol (E2) -BSA (bovine serum albumin)-FITC (fluorescein isothiocyanate) macromolecular complex was found to selectively label MCF-7, potentially serving as a cancer cell detection marker. MCF-7 cells were detected with specific and strong FITC signals after only 4 min of contact with the stain. The signals were about seven times stronger than that of a labeling performed on conventional glass slides. These results strongly suggest that this novel design has a potential application to detect cancer cells or other diseased cells without compromising the advantage of high sensitivity of the microfluidic approach. © 2009 American Vacuum Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1116/1.3049529
dc.sourceScopus
dc.typeConference Paper
dc.contributor.departmentELECTRICAL & COMPUTER ENGINEERING
dc.description.doi10.1116/1.3049529
dc.description.sourcetitleJournal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures
dc.description.volume27
dc.description.issue3
dc.description.page1295-1298
dc.description.codenJVTBD
dc.identifier.isiut000266500300054
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