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|Title:||Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay||Authors:||Vashist, S.K.
|Issue Date:||Oct-2011||Citation:||Vashist, S.K., Zhang, B., Zheng, D., Al-Rubeaan, K., Luong, J.H.T., Sheu, F.-S. (2011-10). Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay. Analytical Biochemistry 417 (1) : 156-158. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ab.2011.05.045||Abstract:||This study revealed a major interference from sulfo-N-hydroxysuccinimide (sulfo-NHS) in the bicinchoninic acid (BCA) protein assay. Sulfo-NHS, a common reagent used in bioconjugation and analytical biochemistry, exhibited absorbance signals and absorbance peaks at 562 nm, comparable to bovine serum albumin (BSA). However, the combined absorbance of sulfo-NHS and BSA was not strictly additive. The sulfo-NHS interference was suggested to be caused by the reduction of Cu 2+ in the BCA Kit's reagent B (4% cupric sulfate) in a manner similar to that of the protein. Crown Copyright © 2011 Published by Elsevier Inc. All rights reserved.||Source Title:||Analytical Biochemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/83113||ISSN:||00032697||DOI:||10.1016/j.ab.2011.05.045|
|Appears in Collections:||Staff Publications|
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