Please use this identifier to cite or link to this item: https://doi.org/10.1166/jbn.2014.1836
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dc.titleNanoscale helium ion microscopic analysis of collagen fibrillar changes following femtosecond laser dissection of human cornea
dc.contributor.authorRiau, A.K.
dc.contributor.authorPoh, R.
dc.contributor.authorPickard, D.S.
dc.contributor.authorPark, C.H.J.
dc.contributor.authorChaurasia, S.S.
dc.contributor.authorMehta, J.S.
dc.date.accessioned2014-10-07T04:32:58Z
dc.date.available2014-10-07T04:32:58Z
dc.date.issued2014
dc.identifier.citationRiau, A.K., Poh, R., Pickard, D.S., Park, C.H.J., Chaurasia, S.S., Mehta, J.S. (2014). Nanoscale helium ion microscopic analysis of collagen fibrillar changes following femtosecond laser dissection of human cornea. Journal of Biomedical Nanotechnology 10 (8) : 1552-1562. ScholarBank@NUS Repository. https://doi.org/10.1166/jbn.2014.1836
dc.identifier.issn15507041
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/82742
dc.description.abstractOver the last decade, femtosecond lasers have emerged as an important tool to perform accurate and fine dissections with minimal collateral damage in biological tissue. The most common surgical procedure in medicine utilizing femtosecond laser is LASIK. During the femtosecond laser dissection process, the corneal collagen fibers inevitably undergo biomechanical and thermal changes on a sub-micro- or even a nanoscale level, which can potentially lead to post-surgical complications. In this study, we utilized helium ion microscopy, complemented with transmission electron microscopy to examine the femtosecond laser-induced collagen fibrillar damage in ex vivo human corneas. We found that the biomechanical damage induced by laser etching, generation of tissue bridges, and expansion of cavitation bubble and its subsequent collapse, created distortion to the surrounding collagen lamellae. Femtosecond laser-induced thermal damage was characterized by collapsed collagen lamellae, loss of collagen banding, collagen coiling, and presence of spherical debris. Our findings have shown the ability of helium ion microscopy to provide high resolution images with unprecedented detail of nanoscale fibrillar morphological changes in order to assess a tissue damage, which could not be resolved by conventional scanning electron microscopy previously. This imaging technology has also given us a better understanding of the tissue-laser interactions in a nano-structural manner and their possible effects on post-operative wound recovery. Copyright © 2014 American Scientific Publishers All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1166/jbn.2014.1836
dc.sourceScopus
dc.subjectCavitation bubble
dc.subjectCollagen
dc.subjectCornea
dc.subjectFemtosecond laser
dc.subjectHelium ion microscopy
dc.subjectLASIK
dc.subjectPhotodisruption
dc.subjectPlasma
dc.typeArticle
dc.contributor.departmentELECTRICAL & COMPUTER ENGINEERING
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1166/jbn.2014.1836
dc.description.sourcetitleJournal of Biomedical Nanotechnology
dc.description.volume10
dc.description.issue8
dc.description.page1552-1562
dc.identifier.isiut000334414000015
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