Please use this identifier to cite or link to this item: https://doi.org/10.1002/ppsc.201300111
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dc.titleReciprocal response of human oral epithelial cells to internalized silica nanoparticles
dc.contributor.authorTay, C.Y.
dc.contributor.authorFang, W.
dc.contributor.authorSetyawati, M.I.
dc.contributor.authorSum, C.P.
dc.contributor.authorXie, J.
dc.contributor.authorNg, K.W.
dc.contributor.authorChen, X.
dc.contributor.authorHong, C.H.L.
dc.contributor.authorLeong, D.T.
dc.date.accessioned2014-09-18T10:00:54Z
dc.date.available2014-09-18T10:00:54Z
dc.date.issued2013-09
dc.identifier.citationTay, C.Y., Fang, W., Setyawati, M.I., Sum, C.P., Xie, J., Ng, K.W., Chen, X., Hong, C.H.L., Leong, D.T. (2013-09). Reciprocal response of human oral epithelial cells to internalized silica nanoparticles. Particle and Particle Systems Characterization 30 (9) : 784-793. ScholarBank@NUS Repository. https://doi.org/10.1002/ppsc.201300111
dc.identifier.issn09340866
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/79975
dc.description.abstractSilica nanoparticles (SiO2 NPs) are one of the most widely used engineered nanoparticles and can been found in a wide range of consumer products. Despite their massive global production scale, little is known about their potential effects in the context of unintended exposure or ingestion. Using TR146 cells as an in vitro model of the human oral buccal mucosa, the uptake, spatial intracellular distribution, reactive oxygen species (ROS) production, inflammatory response, and cytotoxic effects of commercial SiO 2 NPs are examined. SiO2 NPs are shown to dock and cross the cellular membrane barrier in a dose-time-dependent manner. Confocal sectioning reveals translocation of SiO2 NPs into the cell nucleus after 12 h of exposure. A concentration threshold of more than 500 × 10-6 m is observed, above which SiO2 NPs are shown to exert significant oxidative stress with concomitant upregulation of inflammatory genes IL6 and TNFA. Further analysis of the p53 pathway and a series of apoptotic and cell cycle biomarkers reveals intracellular accumulation of SiO2 NPs exert marginal nanotoxicity. Collectively, this study provides important information regarding the uptake, intracellular distribution, and potential adverse cellular effects of SiO2 NPs commonly found in consumer products in the human oral epithelium. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/ppsc.201300111
dc.sourceScopus
dc.subjecthuman buccal epithelium
dc.subjectinflammation
dc.subjectnanomaterials
dc.subjectreactive oxygen species
dc.subjectsilica nanoparticles
dc.typeArticle
dc.contributor.departmentDENTISTRY
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1002/ppsc.201300111
dc.description.sourcetitleParticle and Particle Systems Characterization
dc.description.volume30
dc.description.issue9
dc.description.page784-793
dc.description.codenPPCHE
dc.identifier.isiut000327250600008
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