Please use this identifier to cite or link to this item: https://doi.org/10.1089/scd.2007.0088
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dc.titleHuman embryonic stem cells may display higher resistance to genotoxic stress as compared to primary explanted somatic cells
dc.contributor.authorVinoth, K.J.
dc.contributor.authorHeng, B.C.
dc.contributor.authorPoonepalli, A.
dc.contributor.authorBanerjee, B.
dc.contributor.authorBalakrishnan, L.
dc.contributor.authorLu, K.
dc.contributor.authorHande, M.P.
dc.contributor.authorCao, T.
dc.date.accessioned2014-09-18T10:00:50Z
dc.date.available2014-09-18T10:00:50Z
dc.date.issued2008-06-01
dc.identifier.citationVinoth, K.J., Heng, B.C., Poonepalli, A., Banerjee, B., Balakrishnan, L., Lu, K., Hande, M.P., Cao, T. (2008-06-01). Human embryonic stem cells may display higher resistance to genotoxic stress as compared to primary explanted somatic cells. Stem Cells and Development 17 (3) : 599-607. ScholarBank@NUS Repository. https://doi.org/10.1089/scd.2007.0088
dc.identifier.issn15473287
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/79969
dc.description.abstractThe use of human embryonic stem (hES) cells in genotoxicity screening can potentially overcome the deficiencies associated with using immortalized cell lines, primary explanted somatic cells, and live animal models. Hence this study sought to compare the responses of hES cells and primary explanted somatic cells (IMR-90 cells, human fetal lung fibroblasts) to genotoxic stress, to evaluate whether hES cells can accurately reflect the normal physiology of human somatic cells. The effects of mitomycin C (MMC) on the chromosomal stability of hESC and IMR-90 was assayed and compared by fluorescence in situ hybridization (FISH) with telomere-specific peptide nucleic acid and multicolor (m) FISH techniques. The results showed that, the percentage of aberrant cells increased from 6% in the untreated control to 57.5% at the higher dose of 0.06 μg/ml MMC (9.6-fold increase) group in the case of IMR-90 cells, whereas hES cells displayed a corresponding increase from 6% to 28% (4.6-fold increase). Telomere FISH ascertained that the main types of damage induced by MMC are chromosomal breaks and the loss of telomeric signals. No fusions were observed in all samples analyzed. This was further confirmed by mFISH, which showed that fusions and translocations were not the type of aberration induced by MMC, with no such aberrations being observed in all samples analyzed. Hence, hES cells of the H1 line are apparently more resistant to MMC-induced DNA damage, as compared to the IMR-90 cells. These results highlight possible intrinsic differences in response to damaging agents between hES cells and normal somatic cells. © Mary Ann Liebert, Inc. 2008.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1089/scd.2007.0088
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentORAL AND MAXILLOFACIAL SURGERY
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1089/scd.2007.0088
dc.description.sourcetitleStem Cells and Development
dc.description.volume17
dc.description.issue3
dc.description.page599-607
dc.description.codenSCDTA
dc.identifier.isiut000257115600020
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