Please use this identifier to cite or link to this item:
https://doi.org/10.1007/s10540-007-9051-2
DC Field | Value | |
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dc.title | Caspase inhibitor Z-VAD-FMK enhances the freeze-thaw survival rate of human embryonic stem cells | |
dc.contributor.author | Heng, B.C. | |
dc.contributor.author | Clement, M.V. | |
dc.contributor.author | Cao, T. | |
dc.date.accessioned | 2014-09-18T10:00:41Z | |
dc.date.available | 2014-09-18T10:00:41Z | |
dc.date.issued | 2007-10 | |
dc.identifier.citation | Heng, B.C., Clement, M.V., Cao, T. (2007-10). Caspase inhibitor Z-VAD-FMK enhances the freeze-thaw survival rate of human embryonic stem cells. Bioscience Reports 27 (4-5) : 257-264. ScholarBank@NUS Repository. https://doi.org/10.1007/s10540-007-9051-2 | |
dc.identifier.issn | 01448463 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/79957 | |
dc.description.abstract | Previous study demonstrated that the low survival of human embryonic stem cells (hESC) under conventional slow-cooling cryopreservation protocols is predominantly due to apoptosis rather than cellular necrosis. Hence, this study investigated whether a synthetic broad-spectrum irreversible inhibitor of caspase enzymes, Z-VAD-FMK can be used to enhance the post-thaw survival rate of hESC. About 100 mM Z-VAD-FMK was supplemented into either the freezing solution, the post-thaw culture media or both. Intact and adherent hESC colonies were cryopreserved so as to enable subsequent quantitation of the post-thaw cell survival rate through the MTT assay, which can only be performed with adherent cells. Exposure to 100 mM Z-VAD-FMK in the freezing solution alone did not significantly enhance the post-thaw survival rate (10.2% vs. 9.9%, p > 0.05). However, when 100 mM Z-VAD-FMK was added to the post-thaw culture media, there was a significant enhancement in the survival rate from 9.9% to 14.4% (p < 0.05), which was further increased to 18.7% when Z-VAD-FMK was also added to the freezing solution as well (p < 0.01). Spontaneous differentiation of hESC after cryopreservation was assessed by morphological observations under bright-field microscopy, and by immunocytochemical staining for the pluripotency markers SSEA-3 and TRA-1-81. The results demonstrated that exposure to Z-VAD-FMK did not significantly enhance the spontaneous differentiation of hESC within post-thaw culture. © 2007 The Biochemical Society. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s10540-007-9051-2 | |
dc.source | Scopus | |
dc.subject | Apoptosis | |
dc.subject | Cryopreservation | |
dc.subject | Embryonic | |
dc.subject | Freezing | |
dc.subject | Human | |
dc.subject | Inhibition | |
dc.subject | Stem cells | |
dc.type | Article | |
dc.contributor.department | ORAL AND MAXILLOFACIAL SURGERY | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1007/s10540-007-9051-2 | |
dc.description.sourcetitle | Bioscience Reports | |
dc.description.volume | 27 | |
dc.description.issue | 4-5 | |
dc.description.page | 257-264 | |
dc.description.coden | BRPTD | |
dc.identifier.isiut | 000248762300005 | |
Appears in Collections: | Staff Publications |
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